Sajadi Elaheh, Babaipour Valiollah, Deldar Ali Asghar, Yakhchali Bagher, Fatemi Seyed Safa-Ali
Department of Systems Biotechnology, Institute of Industrial and Environmental Biotechnology (IIEB), National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, P.O. Box: 14965/161, Iran.
Malek Ashtar University of Technology, Tehran, Iran.
Biotechnol Lett. 2017 Sep;39(9):1395-1401. doi: 10.1007/s10529-017-2366-6. Epub 2017 Jun 1.
To evaluate the crystallinity index of the cellulose produced by Escherichia coli Nissle 1917 after heterologous expression of the cellulose synthase subunit D (bcsD) gene of Gluconacetobacter xylinus BPR2001.
The bcsD gene of G. xylinus BPR2001 was expressed in E. coli and its protein product was visualized using SDS-PAGE. FTIR analysis showed that the crystallinity index of the cellulose produced by the recombinants was 0.84, which is 17% more than that of the wild type strain. The increased crystallinity index was also confirmed by X-ray diffraction analysis. The cellulose content was not changed significantly after over-expressing the bcsD.
The bcsD gene can improve the crystalline structure of the bacterial cellulose but there is not any significant difference between the amounts of cellulose produced by the recombinant and wild type E. coli Nissle 1917.
评估在大肠杆菌Nissle 1917中异源表达木醋杆菌BPR2001的纤维素合酶亚基D（bcsD）基因后所产生纤维素的结晶度指数。
木醋杆菌BPR2001的bcsD基因在大肠杆菌中表达，其蛋白质产物通过SDS-PAGE进行可视化。傅里叶变换红外光谱（FTIR）分析表明，重组体产生的纤维素的结晶度指数为0.84，比野生型菌株高17%。X射线衍射分析也证实了结晶度指数的增加。过表达bcsD后纤维素含量没有显著变化。
bcsD基因可以改善细菌纤维素的晶体结构，但重组大肠杆菌Nissle 1917与野生型所产生的纤维素量之间没有任何显著差异。
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