[Regulating the structure of bacterial cellulose by altering the expression of using CRISPR/dCas9].

is a primary strain producing bacterial cellulose (BC). In , BcsD is a subunit of cellulose synthase and is participated in the assembly process of BC. A series of with different expression levels of the gene were obtained by using the CRISPR/dCas9 technique. Analysis of the structural characteristics of BC showed that the crystallinity and porosity of BC changed with the expression of . The porosity varied from 59.95%-84.05%, and the crystallinity varied from 74.26%-93.75%, while the yield of BC did not decrease significantly upon changing the expression levels of . The results showed that the porosity of bacterial cellulose significantly increased, while the crystallinity was positively correlated with the expression of , when the expression level of was below 55.34%. By altering the expression level of the gene, obtaining BC with different structures but stable yield through a one-step fermentation of was achieved.