通过免疫沉淀法对重组埃博拉病毒核衣壳中的RNA含量进行定量分析。

Quantification of RNA Content in Reconstituted Ebola Virus Nucleocapsids by Immunoprecipitation.

作者信息

Banadyga Logan, Ebihara Hideki

机构信息

Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA.

Department of Molecular Medicine, Mayo Clinic, Rochester, MN, USA.

出版信息

Methods Mol Biol. 2017;1628:93-107. doi: 10.1007/978-1-4939-7116-9_7.

DOI:10.1007/978-1-4939-7116-9_7

PMID:28573613

Abstract

Immunoprecipitations are commonly used to isolate proteins or protein complexes and assess protein-protein interactions; however, they can also be used to assess protein-RNA complexes. Here we describe an adapted RNA immunoprecipitation technique that permits the quantification of RNA content in Ebola virus nucleocapsids that have been reconstituted in vitro by transient transfection.

摘要

免疫沉淀法通常用于分离蛋白质或蛋白质复合物，并评估蛋白质-蛋白质相互作用；然而，它也可用于评估蛋白质-RNA复合物。在这里，我们描述了一种改良的RNA免疫沉淀技术，该技术能够对通过瞬时转染在体外重建的埃博拉病毒核衣壳中的RNA含量进行定量。