Department of Dairy Science, University of Wisconsin-Madison, Madison, WI, USA.
Endocrinology & Reproductive Physiology Program, University of Wisconsin-Madison, Madison, WI, USA.
Biol Reprod. 2017 Oct 1;97(4):550-563. doi: 10.1093/biolre/iox050.
Seventeen nonlactating Holstein cows were superovulated in a Latin-square designed experiment to determine the effects of increased propylene glycol (PROP) and luteinizing hormone (LH) during antral follicle development on ovarian function, fertilization, and early embryo quality. PROP was orally drenched every 4 h for 7 days to induce hyperinsulinemia and associated metabolic changes. LH concentrations were altered by increasing LH (3-fold) during last 2 days of superovulation. Treatment groups were as follows: (1) control-oral drenching with water plus low-LH preparation; (2) high LH(HLH)-water plus HLH preparation; (3) PROP-drenching with PROP plus low LH; (4) PROP/HLH-PROP plus HLH. PROP increased glucose (P < 0.05) and insulin (P < 0.02) concentrations at all time points analyzed. Neither PROP nor LH affected numbers of follicles > 9 mm at time of gonadotropin-releasing hormone-induced LH surge, although percentage of these follicles that ovulated was decreased by both PROP (P = 0.002) and LH (P = 0.048). In addition, PROP tended (P = 0.056) to decrease total number of ovulations. PROP reduced (P = 0.028) fertilization rate, while LH tended (P = 0.092) to increase fertilization rate. There was no effect of either PROP or LH on any measure of embryo quality including percentage of embryos that were degenerate, quality 1, or quality 1 and 2 of total structures collected or fertilized structures. These results indicate that acute elevation in insulin during the preovulatory follicular wave can decrease percentage of large follicles that ovulate, particularly when combined with increased LH, and reduce fertilization of ovulated oocytes.
17 头非泌乳荷斯坦奶牛接受了拉丁方设计实验的超数排卵，以确定在窦卵泡发育过程中增加丙二醇（PROP）和促黄体激素（LH）对卵巢功能、受精和早期胚胎质量的影响。PROP 通过每天口服浸渍 4 小时来诱导高胰岛素血症和相关的代谢变化。在超数排卵的最后 2 天增加 LH（3 倍）来改变 LH 浓度。处理组如下：（1）对照-口服水加低 LH 制剂；（2）高 LH（HLH）-水加 HLH 制剂；（3）PROP 浸渍-PROP 加低 LH；（4）PROP/HLH-PROP 加 HLH。PROP 增加了葡萄糖（P<0.05）和胰岛素（P<0.02）在所有分析时间点的浓度。PRO 和 LH 都没有影响 GnRH 诱导 LH 峰时 >9mm 的卵泡数量，尽管 PROP（P=0.002）和 LH（P=0.048）都会降低这些卵泡的排卵率。此外，PROP 还会降低总排卵数（P=0.056）。PROP 降低了受精率（P=0.028），而 LH 则有增加受精率的趋势（P=0.092）。PRO 和 LH 对任何胚胎质量指标都没有影响，包括退化胚胎、1 级胚胎和总结构收集或受精结构的 1 级和 2 级胚胎的比例。这些结果表明，在排卵前卵泡波中急性升高胰岛素会降低排卵的大卵泡比例，尤其是与增加 LH 结合时，并且降低排卵卵母细胞的受精率。
