Cardoso Vanessa F, Sebastián Víctor, Silva Carlos J R, Botelho Gabriela, Lanceros-Méndez Senentxu
Centro de Física, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal; CMEMS-UMinho, Universidade do Minho, DEI, Campus de Azurém, Guimarães 4800-058, Portugal.
Department of Chemical Engineering, Aragon Institute of Nanoscience (INA), University of Zaragoza, Campus Río Ebro-Edificio I + D, C/Poeta Mariano Esquillor S/N, 50018 Zaragoza, Spain; Networking Research Center on Bioengineering, Biomaterials and Nanomedicine CIBER-BBN, Centro de Investigación Biomédica en Red, C/Monforte de Lemos 3-5, Pabellón 11, 28029 Madrid, Spain.
Colloids Surf B Biointerfaces. 2017 Sep 1;157:48-55. doi: 10.1016/j.colsurfb.2017.05.009. Epub 2017 May 5.
Zinc-decorated magnetic silica spheres were developed, optimized and tested for the capture and separation of l-histidine. The magnetic silica spheres were prepared using a simple sol-gel method and show excellent magnetic characteristics, adsorption capacity toward metal ions, and stability in aqueous solution in a wide pH range. The binding capacity of zinc-decorated magnetic silica spheres to histidine proved to be strongly influenced by the morphology, composition and concentration of metal at the surface of the magnetic silica spheres and therefore these parameters should be carefully controlled in order to maximize the performance for protein purification purposes. Optimized zinc-decorated magnetic silica spheres demonstrate a binding capacity to l-histidine of approximately 44mgg at the optimum binding pH buffer.
制备了用于捕获和分离L-组氨酸的锌修饰磁性二氧化硅球，并对其进行了优化和测试。磁性二氧化硅球采用简单的溶胶-凝胶法制备，具有优异的磁特性、对金属离子的吸附能力以及在宽pH范围内水溶液中的稳定性。结果表明，锌修饰磁性二氧化硅球对组氨酸的结合能力受磁性二氧化硅球表面金属的形态、组成和浓度的强烈影响，因此，为了使蛋白质纯化性能最大化，应仔细控制这些参数。优化后的锌修饰磁性二氧化硅球在最佳结合pH缓冲液中对L-组氨酸的结合能力约为44mg/g。
