Sangeetha K, Girija E K
Department of Physics, Periyar University, Salem 636 011, Tamil Nadu, India.
Department of Physics, Periyar University, Salem 636 011, Tamil Nadu, India.
Mater Sci Eng C Mater Biol Appl. 2017 Sep 1;78:1046-1053. doi: 10.1016/j.msec.2017.04.154. Epub 2017 Apr 29.
Preventing implants associated infections is crucial in orthopedics. Local delivery of antibiotics through implants is a promising strategy to solve this issue. In this study, alginate is tailored to control its swelling, entrapment and release of ciprofloxacin (antibiotic) through the formation of interpenetrating polymer network and composite matrices using gelatin and hydroxyapatite. Developed matrices were characterized by Fourier transform infrared spectroscopy, x-ray diffraction and scanning electron microscopy. The individual tailoring approaches exerted significant influence on the swelling behavior of alginate matrix consequently enhanced the drug entrapment and extended the release period. However, the alginate matrix tailored with the combined approaches resulted in a superior matrix, which had a better control over the burst release of ciprofloxacin. Drug release prolonged from 5h to 240h for composite matrix as compared with alginate matrix showing that alginate combined with gelatin and hydroxyapatite sustained the release for longer periods. This matrix revealed excellent biocompatibility with osteoblast like MG-63 cell lines and showed good antibacterial activity against S. aureus and E. coli.
预防植入物相关感染在骨科领域至关重要。通过植入物进行抗生素局部递送是解决这一问题的一种有前景的策略。在本研究中，通过使用明胶和羟基磷灰石形成互穿聚合物网络和复合基质，对藻酸盐进行定制，以控制其膨胀、环丙沙星（抗生素）的包封和释放。通过傅里叶变换红外光谱、X射线衍射和扫描电子显微镜对所制备的基质进行表征。单独的定制方法对藻酸盐基质的膨胀行为产生了显著影响，从而增强了药物包封并延长了释放期。然而，采用组合方法定制的藻酸盐基质产生了一种更优的基质，其对环丙沙星的突释具有更好的控制。与藻酸盐基质相比，复合基质的药物释放时间从5小时延长至240小时，表明藻酸盐与明胶和羟基磷灰石结合可实现更长时间的持续释放。该基质对成骨样MG-63细胞系显示出优异的生物相容性，并对金黄色葡萄球菌和大肠杆菌表现出良好的抗菌活性。
