Kasai Kohei, Kimura Yuka, Miyata Shogo
School of Integrated Design Engineering, Graduate School of Science & Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama, Kanagawa 223-8522, Japan.
Department of Mechanical Engineering, Faculty of Science & Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama, Kanagawa 223-8522, Japan.
Mater Sci Eng C Mater Biol Appl. 2017 Sep 1;78:354-361. doi: 10.1016/j.msec.2017.04.021. Epub 2017 Apr 4.
Culturing pluripotent stem cells effectively requires feeder cell layers or cell adhesion matrix coating. However, the feeder cell layers or animal-derived factors have to be removed to apply the pluripotent stem cells as resources for regenerative medicine. To enable xeno-free culture conditions, we focused on the UV/ozone surface treatment technique for polystyrene cell culture substrates to improve the adhesion and proliferation of pluripotent stem cells. In this study, as a fundamental research for the feeder- and matrix coating-free culture system for embryonic stem cells (ESCs), mouse ESCs were cultured on UV/ozone-modified polystyrene substrates without feeder layers. We observed that UV/ozone surface-modified polystyrene substrates made it possible to culture mESCs under feeder-free conditions without any chemical treatment for the substrates.
有效培养多能干细胞需要饲养层细胞或细胞黏附基质包被。然而，为了将多能干细胞用作再生医学资源，必须去除饲养层细胞或动物源因子。为实现无动物源培养条件，我们聚焦于用于聚苯乙烯细胞培养底物的紫外线/臭氧表面处理技术，以改善多能干细胞的黏附和增殖。在本研究中，作为胚胎干细胞（ESC）无饲养层和无基质包被培养系统的基础研究，小鼠胚胎干细胞在未经饲养层的紫外线/臭氧修饰聚苯乙烯底物上进行培养。我们观察到，紫外线/臭氧表面修饰的聚苯乙烯底物使得在无饲养层条件下培养小鼠胚胎干细胞成为可能，且无需对底物进行任何化学处理。
