Li Kaikai, Cheng Xinran, Jiang Jinhua, Wang Jiutao, Xie Jiongfang, Hu Xinde, Huang Yingxue, Song Lingzhen, Liu Mengmeng, Cai Leiming, Chen Liezhong, Zhao Shanting
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, People's Republic of China.
Zhejiang Academy of Agricultural Science, Hangzhou, Zhejiang 310021, People's Republic of China.
Food Chem Toxicol. 2017 Aug;106(Pt A):356-366. doi: 10.1016/j.fct.2017.05.067. Epub 2017 May 30.
Paraquat, a fast-acting non-selective contact herbicide, is considered an etiological factor related to Parkinson's disease. This study investigated its effects on hippocampal neurogenesis and cognition in adult mice as well as possible mechanisms for the effects. We administered paraquat (1.25 mg/kg, intraperitoneal injection, i.p.) and an equal volume of normal saline for 3 weeks to adult male C57BL/6J mice. The results showed that hippocampus-dependent spatial learning and memory was significantly impaired in paraquat-treated mice. Moreover, paraquat administration inhibited the proliferation of neural progenitor cells, and impaired the survival and altered the fate decision of newly generated cells in the hippocampus. The expression levels of caspase-3 and glial fibrillary acidic protein were significantly higher in paraquat-treated mice than in control mice. Interestingly, paraquat reduced the phosphorylation of Akt, but did not affect the total amount of Akt. In conclusion, our findings suggest that paraquat negatively affected adult hippocampal neurogenesis and cognition function.
百草枯是一种速效非选择性接触性除草剂，被认为是与帕金森病相关的病因。本研究调查了其对成年小鼠海马神经发生和认知的影响以及影响的可能机制。我们对成年雄性C57BL/6J小鼠腹腔注射百草枯（1.25毫克/千克）和等体积的生理盐水，持续3周。结果显示，百草枯处理的小鼠海马依赖性空间学习和记忆显著受损。此外，百草枯给药抑制了神经祖细胞的增殖，损害了新生细胞在海马中的存活并改变了其命运决定。与对照小鼠相比，百草枯处理的小鼠中caspase-3和胶质纤维酸性蛋白的表达水平显著更高。有趣的是，百草枯降低了Akt的磷酸化，但不影响Akt的总量。总之，我们的研究结果表明百草枯对成年海马神经发生和认知功能有负面影响。
