Goldenberg R, Fine R E
Exp Cell Res. 1985 Mar;157(1):41-9. doi: 10.1016/0014-4827(85)90150-8.
Coated vesicles were purified from embryonic chick tendon fibroblasts pulsed with [3H]proline. They were morphologically and biochemically similar to coated vesicles purified from other sources. Furthermore, they contained newly synthesized Type I procollagen which was protected from bacterial collagenase digestion unless detergent was present. The procollagen remained associated with coated vesicles during immune precipitation and agarose gel electrophoresis. Data from pulse-chase experiments demonstrated that the specific activity of the coated vesicle preparations was approx. 5-fold higher at the 10 min chase point than at either the 0 or 40 min chase points. These data are consistent with the hypothesis that coated vesicles are intermediates in the intracellular transport of newly synthesized Type I procollagen in chick tendon fibroblasts.
用[3H]脯氨酸脉冲处理胚胎鸡肌腱成纤维细胞后，从中纯化出被膜小泡。它们在形态和生化方面与从其他来源纯化出的被膜小泡相似。此外，它们含有新合成的I型前胶原，除非存在去污剂，否则该前胶原可免受细菌胶原酶消化。在免疫沉淀和琼脂糖凝胶电泳过程中，前胶原仍与被膜小泡结合。脉冲追踪实验的数据表明，在追踪10分钟时，被膜小泡制剂的比活性比追踪0分钟或40分钟时大约高5倍。这些数据与以下假设一致，即被膜小泡是鸡肌腱成纤维细胞中新生I型前胶原细胞内运输的中间体。
