Department of Nanoscience and Technology, Bharathiar University, Coimbatore, Tamilnadu, 641 046, India.
Department of Environmental Sciences, Bharathiar University, Coimbatore, Tamilnadu, 641 046, India.
Environ Sci Pollut Res Int. 2018 Jul;25(21):20385-20390. doi: 10.1007/s11356-017-9362-1. Epub 2017 Jun 2.
Surface-modified diatom substrates are employed for the development of immunocomplex-based optical biosensor for diagnosis of typhoid. Biosensor has been prepared by covalent immobilization of Salmonella typhi antibody onto the crosslinked diatom substrates via glutaraldehyde. Photoluminescent (PL) studies revealed good specificity and ability of conjugated diatom substrates to distinguish complementary (S. typhi) and non-complementary (Escherichia coli) antigens. The immunocomplexed biosensor showed detection limit of 10 pg. The excellent performance of biosensor is associated to its large surface-to-volume ratio, good photoluminescent property, and biocompatibility of diatom frustules, which enhances the antibody immobilization and facilitates the nucleophilic electron transfer between antibody and conjugated diatom surface. Hence, immunocomplexed diatom substrates are considered to be a suitable platform for the environmental monitoring of water-borne pathogen S. typhi.
表面修饰的硅藻基底被用于开发基于免疫复合物的光学生物传感器，用于伤寒的诊断。生物传感器是通过戊二醛将伤寒沙门氏菌抗体共价固定在交联的硅藻基底上制备的。光致发光（PL）研究表明，共轭硅藻基底具有良好的特异性和区分互补（伤寒沙门氏菌）和非互补（大肠杆菌）抗原的能力。免疫复合物生物传感器的检测限为 10 pg。生物传感器的优异性能与其大的表面积与体积比、良好的光致发光性能以及硅藻壳的生物相容性有关，这增强了抗体的固定化，并促进了抗体与共轭硅藻表面之间的亲核电子转移。因此，免疫复合物化的硅藻基底被认为是用于监测水中病原体伤寒沙门氏菌的合适平台。
