Wu Juan, Wang Chunlan, Zhu Xiwu, Chen Jishuang
Institute of Agriculture and Biotechnology, Hunan University of Humanities, Science and Technology, Loudi, 41700, China.
Institute of Bioengineering, Zhejiang Sci-Tech University, Hangzhou, 310018, China.
Arch Virol. 2017 Sep;162(9):2913-2917. doi: 10.1007/s00705-017-3430-1. Epub 2017 Jun 2.
We have recently reported the identification of 10 double-strand RNA segments from Sclerotium hydrophilum [HZ11] mycelia and of virus-like particles isolated from the mycelia, as well as the sequences of dsRNA2 and dsRNA7. Phylogenetic analysis revealed that dsRNA2 and dsRNA7 belong to a group of unclassified viruses. In this report, we cloned and sequenced dsRNA6 and dsRNA9 from the 10 dsRNAs. We tentatively named the putative virus "Sclerotium hydrophilum virus 1", with isolates being abbreviated to ShV1, with dsRNA6 and dsRNA9 corresponding to dsRNA1 and dsRNA2, respectively, of ShV1. dsRNA1 was 1975 bp in length and encoded a putative RNA-dependent RNA polymerase (RdRp). dsRNA2 was 1728 bp and encoded a putative coat protein (CP). Phylogenetic analysis showed that the proteins encoded by dsRNA1 and dsRNA2 were highly related to known viral RdRps and CP, respectively, of viruses classified within the genus Alphapartitivirus of the family Partitiviridae. These members include Rhizoctonia solani dsRNA virus 2, Diuris pendunculata cryptic virus, and Heterobasidion partitivirus. The 5'- and 3'-untranslated regions (UTRs) of the two dsRNAs showed a high sequence identity. The 5'-UTR contained conserved sequences 5'-GAAGCAUCACUU(/G) G(/U)AGU(/A)UCGC(/U)CCA(/G) CAAUAACGAA-3' and 5'-AAAUUGAUCUUACCUCUCAC-3'. The 3'-UTR contained the conserved sequence 5'-UUGUUUU-3' and 5'-UUUA(/U)A(/C) UUAU-3'. These results indicate that dsRNA1 and dsRNA2 are phylogenetically related to members of the genus Alphapartitivirus of family Partitiviridae. We therefore propose that dsRNA1 and dsRNA2 are the genome sequences of a new partitivirus, ShV1.
我们最近报道了从嗜水小核菌[HZ11]菌丝体中鉴定出10个双链RNA片段以及从菌丝体中分离出的病毒样颗粒，还报道了dsRNA2和dsRNA7的序列。系统发育分析表明，dsRNA2和dsRNA7属于一组未分类的病毒。在本报告中，我们从这10个双链RNA中克隆并测序了dsRNA6和dsRNA9。我们暂定将这种假定的病毒命名为“嗜水小核菌病毒1”，其分离株简称为ShV1，其中dsRNA6和dsRNA9分别对应于ShV1的dsRNA1和dsRNA2。dsRNA1长度为1975 bp，编码一种假定的RNA依赖性RNA聚合酶（RdRp）。dsRNA2长度为1728 bp，编码一种假定的外壳蛋白（CP）。系统发育分析表明，dsRNA1和dsRNA2编码的蛋白质分别与分粒病毒科α分粒病毒属内已分类病毒的已知病毒RdRp和CP高度相关。这些成员包括立枯丝核菌dsRNA病毒-2、垂花双尾兰隐性病毒和异担子菌分粒病毒。这两个双链RNA的5'和3'非翻译区（UTR）显示出高度的序列同一性。5'-UTR包含保守序列5'-GAAGCAUCACUU(/G)G(/U)AGU(/A)UCGC(/U)CCA(/G)CAAUAACGAA-3'和5'-AAAUUGAUCUUACCUCUCAC-3'。3'-UTR包含保守序列5'-UUGUUUU-3'和5'-UUUA(/U)A(/C)UUAU-3'。这些结果表明，dsRNA1和dsRNA2在系统发育上与分粒病毒科α分粒病毒属的成员相关。因此，我们提出dsRNA1和dsRNA2是一种新的分粒病毒ShV1的基因组序列。
