Department of Chemical Engineering, University of Alcalá, E-28871 Alcalá de Henares, Madrid, Spain.
Department of Organic Chemistry and Inorganic Chemistry, School of Biology, Environmental Sciences and Chemistry, University of Alcalá, E-28871 Alcalá de Henares, Madrid, Spain.
Sci Total Environ. 2017 Dec 1;601-602:732-740. doi: 10.1016/j.scitotenv.2017.05.243. Epub 2017 Jun 1.
This work reports the preparation of composite electrospun membranes combining antimicrobial action with the capacity of retaining low-molecular weight non-polar pollutants. The membranes were electrospun blends of polyvinyl alcohol (PVA) and poly(acrylic acid) (PAA) stabilized using heat curing. The membranes were functionalized by grafting amino-terminated poly(amidoamine) (PAMAM) G3 dendrimers. The antimicrobial effect was assessed using strains of Escherichia coli and Staphylococcus aureus by tracking their capacity to form new colonies and their metabolic impairment upon contact with membranes. The antimicrobial activity was particularly high to the gram-positive bacterium S. aureus with a 3-log reduction in their capacity to colonize dendrimer-functionalized membranes with respect to neat PVA/PAA fibers. The effect to gram-positive bacteria was attributed to the interaction of dendrimers with the negatively charged bacterial membranes and resulted in membranes essentially free of bacterial colonization after 20h in contact with cultures at 36°C. The adsorption of toluene on PAA/PVA fibers and on dendrimer-functionalized membranes was assayed using toluene over a broad concentration range. The host-guest encapsulation of toluene inside dendrimer molecules was computed through docking studies, which allowed calculating a maximum capacity of 14 molecules of toluene per molecule of PAMAM G3. The theoretical prediction was in good agreement with the experimental capacity at the higher concentrations assayed.
这项工作报道了一种将抗菌作用与保留低分子量非极性污染物能力相结合的复合电纺膜的制备。该膜是通过热固化稳定的聚乙烯醇（PVA）和聚丙烯酸（PAA）的共混物电纺而成。通过接枝氨基封端的聚（酰胺-胺）（PAMAM）G3 树状大分子对膜进行了功能化。通过跟踪它们形成新菌落的能力以及与膜接触时的代谢损伤，使用大肠杆菌和金黄色葡萄球菌菌株评估了抗菌效果。与纯 PVA/PAA 纤维相比，树状大分子功能化膜对革兰氏阳性菌金黄色葡萄球菌的抗菌活性特别高，其定植能力降低了 3 个对数级。这种对革兰氏阳性菌的作用归因于树状大分子与带负电荷的细菌细胞膜的相互作用，结果是在与 36°C 下的培养物接触 20 小时后，膜基本上没有细菌定植。使用甲苯在较宽的浓度范围内对 PAA/PVA 纤维和树状大分子功能化膜上的甲苯吸附进行了测定。通过对接研究计算了甲苯在树状大分子分子内的主体-客体包封，这允许计算出每个 PAMAM G3 分子可容纳 14 个甲苯分子。理论预测与在较高浓度下测定的实验容量非常吻合。
