Worley Brittany V, Soto Robert J, Kinsley Paige C, Schoenfisch Mark H
Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599.
ACS Biomater Sci Eng. 2016 Mar 14;2(3):426-437. doi: 10.1021/acsbiomaterials.6b00032. Epub 2016 Feb 29.
The fabrication of electrospun composite polyurethane fibers capable of dual-action antibacterial dendrimer release is reported. Generation 4 (G4) poly(amidoamine) dendrimers were functionalized with octyl alkyl chain or quaternary ammonium (QA) moieties followed by modification of the resulting secondary amines with -diazeniumdiolate nitric oxide (NO) donors to produce dual-action antibacterial dendrimers. Control and NO-releasing dendrimers were doped into polyurethane solutions prior to electrospinning of the polymer to yield well-defined dendrimer-doped composite polyurethane fibers. The fiber mats were semi-porous (≥30% porosity) and exhibited high water uptake (>100% relative to fiber mass). Dendrimer- and NO-release characteristics (rates and totals) were dependent on the dendrimer modification and polyurethane composition, with total dendrimer- and NO-release amounts ranging from 10 - 80 μg/mg and 0.027 - 0.072 μmol NO/mg, respectively. The antibacterial action of the fibers was evaluated against Gram-negative and Gram-positive bacterial strains. Nitric oxide-releasing fibers demonstrated broad-spectrum bactericidal action at short (2 h) and long (24 h) timescales.
报道了能够实现双效抗菌树枝状大分子释放的电纺复合聚氨酯纤维的制备。用辛基烷基链或季铵（QA）部分对第4代（G4）聚（酰胺胺）树枝状大分子进行功能化，然后用 - 二氮烯二醇盐一氧化氮（NO）供体对所得仲胺进行改性，以制备双效抗菌树枝状大分子。在对聚合物进行电纺丝之前，将对照树枝状大分子和释放NO的树枝状大分子掺杂到聚氨酯溶液中，以得到定义明确的掺杂树枝状大分子的复合聚氨酯纤维。纤维垫是半多孔的（孔隙率≥30%），并且表现出高吸水性（相对于纤维质量>100%）。树枝状大分子和NO的释放特性（速率和总量）取决于树枝状大分子的改性和聚氨酯组成，树枝状大分子和NO的总释放量分别为10 - 80μg/mg和0.027 - 0.072μmol NO/mg。评估了纤维对革兰氏阴性和革兰氏阳性细菌菌株的抗菌作用。释放一氧化氮的纤维在短时间（2小时）和长时间（24小时）尺度上均表现出广谱杀菌作用。
