Istanbul Technical University, Department of Environmental Engineering, Maslak, 34469 Istanbul, Turkey.
Istanbul Technical University, Department of Environmental Engineering, Maslak, 34469 Istanbul, Turkey.
Bioresour Technol. 2017 Oct;241:332-339. doi: 10.1016/j.biortech.2017.05.070. Epub 2017 May 18.
Performance and microbial community dynamics in an upflow anaerobic sludge bed (UASB) reactor coupled with anaerobic ammonium oxidizing (Anammox) treating diluted chicken manure digestate (Total ammonia nitrogen; TAN=123±10mg/L) were investigated for a 120-d operating period in the presence of anaerobic granular inoculum. Maximum TAN removal efficiency reached to above 80% with as low as 20mg/L TAN concentrations in the effluent. Moreover, total COD (tCOD) with 807±215mg/L in the influent was removed by 60-80%. High-throughput sequencing revealed that Proteobacteria, Actinobacteria, and Firmicutes were dominant phyla followed by Euryarchaeota and Bacteroidetes. The relative abundance of Planctomycetes significantly increased from 4% to 8-9% during the late days of the operation with decreased tCOD concentration, which indicated a more optimum condition to favor ammonia removal through anammox route. There was also significant association between the hzsA gene and ammonia removal in the UASB reactor.
在存在厌氧颗粒接种物的情况下，采用上流式厌氧污泥床（UASB）反应器与厌氧氨氧化（Anammox）处理稀释鸡粪消化液（总氨氮；TAN=123±10mg/L）的组合，对 120d 的运行期内的性能和微生物群落动态进行了研究。在出水中 TAN 浓度低至 20mg/L 时，最大 TAN 去除效率达到 80%以上。此外，进水总 COD（tCOD）为 807±215mg/L，去除率为 60-80%。高通量测序结果表明，在操作后期，随着 tCOD 浓度的降低，Proteobacteria、Actinobacteria 和 Firmicutes 是优势门，其次是 Euryarchaeota 和 Bacteroidetes。随着 tCOD 浓度的降低，浮霉菌的相对丰度从 4%显著增加到 8-9%，这表明通过 Anammox 途径有利于氨去除的条件更加优化。UASB 反应器中 hzsA 基因与氨去除之间也存在显著关联。
