通过同步糖化和共发酵从水稻（Oryza sativa）秸秆生产乙醇。

Ethanol production from Rice (Oryza sativa) straw by simultaneous saccharification and cofermentation.

作者信息

Goel Annu, Wati Leela

出版信息

Indian J Exp Biol. 2016 Aug;54(8):525-9.

Abstract

Ethanol production from alkali treated rice straw was investigated by simultaneous saccharification and cofermentation (SSCF) using commercial cellulase and 3 different yeast strains viz., Saccharomyces cerevisiae HAU-1, Pachysolen tannophilus and Candida sp. individually as well as in combination at varied fermentation temperature and incubation time. Dilute alkali (2%) pretreatment of straw resulted in efficient delignification as observed by low residual lignin (12.52%) with 90.6% cellulose and 28.15% hemicellulose recovery. All the 3 yeast strains were able to produce ethanol form alkali treated rice straw and overall ethanol concentration varied from 5.30 to 24.94 g/L based on different fermentation time and temperature. Comparative analysis of ethanol production from different yeast strains combinations revealed maximum ethanol concentration of 23.48 g/L after 96 h incubation at 35ºC with P. tannophilus individually and 24.94 g/L when used as co-culture with Saccharomyces cerevisiae.

摘要

采用商业纤维素酶以及3种不同酵母菌株，即酿酒酵母HAU-1、嗜单宁管囊酵母和假丝酵母，在不同发酵温度和培养时间下，通过同步糖化共发酵（SSCF）对碱处理稻草生产乙醇进行了研究。稻草经稀碱（2%）预处理后，残余木质素含量低（12.52%），纤维素回收率为90.6%，半纤维素回收率为28.15%，实现了高效脱木质素。所有3种酵母菌株都能够利用碱处理稻草生产乙醇，基于不同的发酵时间和温度，乙醇总浓度在5.30至24.94 g/L之间变化。对不同酵母菌株组合生产乙醇的比较分析表明，在35ºC下单独培养嗜单宁管囊酵母96小时后，乙醇最高浓度为23.48 g/L，与酿酒酵母共培养时乙醇最高浓度为24.94 g/L。