Chadha B S, Kanwar S S, Saini H S, Garcha H S
Department of Microbiology, Guru Nanak Dev University, Amritsar, India.
Acta Microbiol Immunol Hung. 1995;42(1):53-9.
A hybrid process for the fermentation of rice straw hydrolysates into ethanol was designed to simultaneously utilize cellulose and hemicellulose fractions of the agro-residue. The process involved dilute acid hydrolysis (for obtaining C-5 sugars) followed by enzymatic saccharification of cellulose enriched fraction with crude cellulase produced by mixed cultures of Trichoderma reesei Rut C-30 and Aspergillus ochraceus IMI 317911. The fermentation medium containing acid and enzymatic hydrolysate mixture of pentoses and hexoses monomers was fermented with yeast coculture of Saccharomyces cerevisiae and Pachysolen tannophilus resulting in 295.0 ml ethanol/kg of rice straw. The hybrid process resulted in an efficient utilization of both cellulosic and hemicellulosic components of the rice straw for ethanol production.
设计了一种将稻草水解产物发酵成乙醇的混合工艺，以同时利用这种农业残留物中的纤维素和半纤维素部分。该工艺包括稀酸水解（用于获得C-5糖），然后用里氏木霉Rut C-30和赭曲霉IMI 317911混合培养产生的粗纤维素酶对富含纤维素的部分进行酶促糖化。含有戊糖和己糖单体的酸和酶水解产物混合物的发酵培养基用酿酒酵母和嗜单宁管囊酵母的酵母共培养物进行发酵，每千克稻草产生295.0毫升乙醇。这种混合工艺有效地利用了稻草中的纤维素和半纤维素成分来生产乙醇。
