用Aβ3-10-KLH疫苗免疫Tg-APPswe/PSEN1dE9小鼠可预防阿尔茨海默病的突触缺陷。
Immunization of Tg-APPswe/PSEN1dE9 mice with Aβ3-10-KLH vaccine prevents synaptic deficits of Alzheimer's disease.
作者信息
Meng Yuan, Ding Li, Zhang HuiYi, Yin WenChao, Yan Yi, Cao YunPeng
机构信息
Department of Neurology, The First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China.
Department of Neurology, The First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China.
出版信息
Behav Brain Res. 2017 Aug 14;332:64-70. doi: 10.1016/j.bbr.2017.05.056. Epub 2017 May 31.
The amyloid cascade hypothesis is widely accepted by researchers as the mechanism of Alzheimer's disease. Active immunization to eliminate Aβ depositions has been used in preclinical and clinical studies. Aβ3-10-KLH is a vaccine of the Aβ3-10 peptide combined with keyhole limpet hemocyanin (KLH). We immunized 2.5-month-old Tg-APPswe/PSEN1dE9 mice with Aβ3-10-KLH peptide and Aβ1-42 peptide to eliminate Aβ deposits in the brains of these transgenic mice. The results of immunohistochemical staining and transmission electron microscopy detection indicated that our new vaccine was more advantageous in protecting synaptic function and structure than the Aβ1-42 peptide. Aβ3-10-KLH peptide is an effective vaccine in the treatment of Alzheimer's disease.
淀粉样蛋白级联假说作为阿尔茨海默病的发病机制被研究人员广泛接受。旨在消除Aβ沉积的主动免疫已用于临床前和临床研究。Aβ3-10-KLH是一种由Aβ3-10肽与钥孔血蓝蛋白（KLH）结合而成的疫苗。我们用Aβ3-10-KLH肽和Aβ1-42肽对2.5月龄的Tg-APPswe/PSEN1dE9小鼠进行免疫，以消除这些转基因小鼠大脑中的Aβ沉积。免疫组织化学染色和透射电子显微镜检测结果表明，我们的新型疫苗在保护突触功能和结构方面比Aβ1-42肽更具优势。Aβ3-10-KLH肽是治疗阿尔茨海默病的一种有效疫苗。
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