Ovryn Ben, Li Jie, Hong Senlian, Wu Peng
Department of Molecular Medicine, The Scripps Research Institute, 10550 N Torrey Pines Rd, La Jolla, CA 92037, United States.
Department of Molecular Medicine, The Scripps Research Institute, 10550 N Torrey Pines Rd, La Jolla, CA 92037, United States.
Curr Opin Chem Biol. 2017 Aug;39:39-45. doi: 10.1016/j.cbpa.2017.04.018. Epub 2017 Jun 1.
Metabolic oligosaccharide engineering and chemoenzymatic glycan labeling have provided powerful tools to study glycans in living systems and tissue samples. In this review article, we summarize recent advances in this field with a focus on innovative approaches for glycan imaging. The presented applications demonstrate that several of the leading imaging methods, which have revolutionized quantitative cell biology, can be adapted to imaging glycans on single cells and tissues.
代谢寡糖工程和化学酶促聚糖标记为研究活细胞系统和组织样本中的聚糖提供了强大工具。在这篇综述文章中,我们总结了该领域的最新进展,重点关注聚糖成像的创新方法。所展示的应用表明,一些引领性的成像方法已经彻底改变了定量细胞生物学,并且可以适用于单细胞和组织上聚糖的成像。