Pan R, Liu X, Zhou S, Ning Z, Zheng H, Gao M, Ding Y, Yao W, Liao X, He N
Department of Epidemiology,School of Public Health, and the Key Laboratory of Public Health Safety of Ministry of Education, Fudan University,Shanghai,China.
Shanghai Center for Disease Control and Prevention,Shanghai,China.
Epidemiol Infect. 2017 Aug;145(11):2330-2340. doi: 10.1017/S0950268817001054. Epub 2017 Jun 5.
This cross-sectional study aimed to examine and compare prevalence and correlates of whole blood Epstein-Barr virus (EBV) DNA between HIV-positive and HIV-negative men who have sex with men (MSM). Five hundred and four HIV-positive MSM and 504 age-matched HIV-negative MSM were recruited from an HIV counseling and testing clinic in Shanghai, China from November 2014 to November 2015 and were administered with a face-to-face questionnaire interview. Whole blood EBV DNA was tested by nested polymerase chain reaction assays on EBNA-1, EBNA-2, and LMP-1 genes. The prevalence of whole blood EBV DNA was 56·0% (95% CI 51·7-60·3%) among HIV-positive MSM and 26·0% (95% CI 22·4-30·0%) among HIV-negative MSM. Whole blood EBV DNA positivity was significantly associated with HIV infection (adjusted odds ratio (aOR) 3·43, 95% CI 2·58-4·57) and frequent intake of pickled, smoked, or salty food (aOR 1·71, 95% CI 1·02-2·86) in the whole sample, and with <200 cells/μl CD4 cell counts (aOR 1·79, 95% CI 1·05-3·05) and pickled, smoked, or salty food intake (aOR 3·14, 95% CI 1·39-7·08) in HIV-positive group. HIV-infected MSM are at higher risk of active EBV replication than HIV-uninfected MSM, underscoring needs of surveillance and research on EBV-related carcinogenesis in this population.
这项横断面研究旨在检测和比较男男性行为者（MSM）中，HIV阳性与HIV阴性个体之间全血中爱泼斯坦-巴尔病毒（EBV）DNA的流行率及其相关因素。2014年11月至2015年11月期间，从中国上海一家HIV咨询检测诊所招募了504名HIV阳性MSM和504名年龄匹配的HIV阴性MSM，并对他们进行了面对面问卷调查。采用巢式聚合酶链反应检测全血中EBV DNA的EBNA-1、EBNA-2和LMP-1基因。HIV阳性MSM中全血EBV DNA的流行率为56.0%（95%CI 51.7-60.3%），HIV阴性MSM中为26.0%（95%CI 22.4-30.0%）。在整个样本中，全血EBV DNA阳性与HIV感染（校正比值比（aOR）3.43，95%CI 2.58-4.57）以及经常食用腌制、烟熏或咸味食物（aOR 1.71，95%CI 1.02-2.86）显著相关；在HIV阳性组中，全血EBV DNA阳性与CD4细胞计数<200个/μl（aOR 1.79，95%CI 1.05-3.05）以及食用腌制、烟熏或咸味食物（aOR 3.14，95%CI 1.39-7.08）显著相关。与未感染HIV的MSM相比，感染HIV的MSM发生EBV活跃复制的风险更高，这突出了对该人群中EBV相关致癌作用进行监测和研究的必要性。
