Centro de Investigaciones y Transferencia (CONICET), Universidad Nacional de Villa María, Villa María, Córdoba, Argentina; Departamento de Química, Universidad Nacional de Río Cuarto, Agencia Postal # 3, C.P. X5804BYA Río Cuarto, Argentina.
Centro de Investigaciones y Transferencia (CONICET), Universidad Nacional de Villa María, Villa María, Córdoba, Argentina.
Carbohydr Polym. 2017 Sep 1;171:85-93. doi: 10.1016/j.carbpol.2017.04.074. Epub 2017 May 2.
It is known that Chitosan (Ch) can be used in several applications, such as antimicrobial agent or as drug delivery agent. However, being its water dispersibility very low at physiological pH it is necessary to find a way to improve it. One attractive strategy is to synthesize Chitosan Nanoparticles (Ch-NPs). In this work, a versatile method to obtain Ch-NPs with different and controlled sizes, that were successfully prepared by cross-linking reaction of glutaraldehyde and native chitosan inside of n-heptane/sodium 1,4-bis-2-ethylhexylsulfosuccinate (AOT)/water reverse micelles (RMs) is presented. Highly monodisperse NPs were synthesized as confirmed by Dynamic Light Scattering (DLS) and Scanning Electron Microscopy (SEM) techniques. The particle size was dependent on the reactants concentration, cross-linking degree and mainly the amount of water inside of the AOT RMs used as nanoreactors. While the cross-linking is quite difficult to control in bulk water, the reaction inside the RMs is more manageable and efficient.
已知壳聚糖（Ch）可用于多种应用，如抗菌剂或药物递送剂。然而，由于其在生理 pH 值下的水分散性非常低，因此有必要找到一种方法来改善它。一种有吸引力的策略是合成壳聚糖纳米粒子（Ch-NPs）。在这项工作中，提出了一种通用的方法来获得具有不同和可控尺寸的 Ch-NPs，该方法通过在正庚烷/十二烷基硫酸钠 1,4-双（2-乙基己基）磺酸钠（AOT）/水反胶束（RMs）内部通过戊二醛和天然壳聚糖的交联反应成功制备。通过动态光散射（DLS）和扫描电子显微镜（SEM）技术证实了高度单分散性的 NPs 的合成。颗粒尺寸取决于反应物浓度、交联度，主要取决于用作纳米反应器的 AOT RMs 内的水量。虽然在本体水中交联很难控制，但在 RMs 内部的反应更易于控制且更高效。
