Wong Louise H, Čopič Alenka, Levine Tim P
UCL Institute of Ophthalmology, 11-43 Bath Street, London EC1V 9EL, UK.
Institut Jacques Monod, CNRS, UMR 7592, Université Paris Diderot, Sorbonne Paris Cité, F-75013 Paris, France.
Trends Biochem Sci. 2017 Jul;42(7):516-530. doi: 10.1016/j.tibs.2017.05.001. Epub 2017 Jun 1.
Transfer of lipid across the cytoplasm is an essential process for intracellular lipid traffic. Lipid transfer proteins (LTPs) are defined by highly controlled in vitro experiments. The functional relevance of these is supported by evidence for the same reactions inside cells. Major advances in the LTP field have come from structural bioinformatics identifying new LTPs, and from the development of countercurrent models for LTPs. However, the ultimate aim is to unite in vitro and in vivo data, and this is where much progress remains to be made. Even where in vitro and in vivo experiments align, rates of transfer tend not to match. Here we set out some of the advances that might test how LTPs work.
脂质穿过细胞质的转运是细胞内脂质运输的一个重要过程。脂质转运蛋白（LTPs）是通过高度可控的体外实验定义的。细胞内相同反应的证据支持了这些蛋白的功能相关性。LTP领域的主要进展来自于通过结构生物信息学鉴定新的LTPs，以及LTPs逆流模型的发展。然而，最终目标是将体外和体内数据结合起来，而这正是仍有待取得很大进展的地方。即使体外和体内实验结果一致，转运速率往往也不匹配。在这里，我们阐述了一些可能用于测试LTPs工作方式的进展。
