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多发性骨髓瘤患者和正常个体骨髓间充质干细胞中 和 的表达

Expression of and in Bone Marrow Derived Mesenchymal Stem Cells from Multiple Myeloma Patients and Normal Individuals.

作者信息

Mansurabadi Raziyeh, Abroun Saeid, Hajifathali Abass, Asri Amir, Atashi Amir, Haghighi Mansoureh

机构信息

Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

Bone Marrow Transplantation Center, Taleghani Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

出版信息

Cell J. 2017 Spring;19(Suppl 1):27-36. doi: 10.22074/cellj.2017.4480. Epub 2017 May 17.

DOI:10.22074/cellj.2017.4480
PMID:28580305
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5448321/
Abstract

OBJECTIVE

Multiple Myeloma (MM) is a heterogeneous cytogenetic disorder in which clonal plasma cells proliferate in the bone marrow (BM) and cause bone destruction. The BM microenvironment plays a crucial role in pathogenesis of this disease, and mesenchymal stem cells (MSCs) are one of the key players. Herein, we propose to investigate the expressions of , runt-related transcription factor 2 (), peroxisome proliferator-activated receptor gamma (), and B-cell lymphoma 2 () as factors involved in osteogenesis, adipogenesis, and MSC survival in BM-MSCs from MM patients and normal individuals.

MATERIALS AND METHODS

In this experimental study, we isolated MSCs from BM aspirates of MM patients and healthy donors. Total RNA were extracted before and after co-culture with L363 myeloma cells. Gene expressions of , , , and were assessed by quantitive real time polymerase chain reaction (qRT-PCR).

RESULTS

Higher levels of , , and expressions existed in MM- MSCs compared to normally derived (ND)-MSCs. expression decreased in MM- MSCs. We observed different results in the co-culture model.

CONCLUSION

In general, the MM-MSCs gene expression profile differed compared to ND- MSCs. Upregulation of , , and in MM-MSCs compared to ND- MSCs would result in formation of bone defects. Downregulation of would lead to MM-MSC cell death.

摘要

目的

多发性骨髓瘤（MM）是一种异质性细胞遗传学疾病，其中克隆性浆细胞在骨髓（BM）中增殖并导致骨质破坏。骨髓微环境在该疾病的发病机制中起关键作用，间充质干细胞（MSCs）是关键参与者之一。在此，我们提议研究 runt 相关转录因子 2（Runx2）、过氧化物酶体增殖物激活受体γ（PPARγ）和 B 细胞淋巴瘤 2（Bcl-2）作为参与 MM 患者和正常个体骨髓间充质干细胞（BM-MSCs）成骨、脂肪生成和 MSC 存活的因子的表达情况。

材料与方法

在本实验研究中，我们从 MM 患者和健康供体的骨髓抽吸物中分离出 MSCs。在与 L363 骨髓瘤细胞共培养前后提取总 RNA。通过定量实时聚合酶链反应（qRT-PCR）评估 Runx2、PPARγ、Bcl-2 和 Bcl-xL 的基因表达。

结果

与正常来源（ND）的 MSCs 相比，MM-MSCs 中 Runx2、PPARγ 和 Bcl-2 的表达水平更高。Bcl-xL 在 MM-MSCs 中的表达降低。我们在共培养模型中观察到了不同的结果。

结论

总体而言，与 ND-MSCs 相比，MM-MSCs 的基因表达谱有所不同。与 ND-MSCs 相比，MM-MSCs 中 Runx2、PPARγ 和 Bcl-2 的上调会导致骨缺损的形成。Bcl-xL 的下调会导致 MM-MSC 细胞死亡。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e0e/5448321/6a881d4ce935/Cell-J-19-Suppl1-27-g07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e0e/5448321/72569d56a3f4/Cell-J-19-Suppl1-27-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e0e/5448321/65f6b4740edc/Cell-J-19-Suppl1-27-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e0e/5448321/d91c4af39b80/Cell-J-19-Suppl1-27-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e0e/5448321/786e083a3f50/Cell-J-19-Suppl1-27-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e0e/5448321/1c98e980422e/Cell-J-19-Suppl1-27-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e0e/5448321/acbba0c2a57c/Cell-J-19-Suppl1-27-g06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e0e/5448321/6a881d4ce935/Cell-J-19-Suppl1-27-g07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e0e/5448321/72569d56a3f4/Cell-J-19-Suppl1-27-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e0e/5448321/65f6b4740edc/Cell-J-19-Suppl1-27-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e0e/5448321/d91c4af39b80/Cell-J-19-Suppl1-27-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e0e/5448321/786e083a3f50/Cell-J-19-Suppl1-27-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e0e/5448321/1c98e980422e/Cell-J-19-Suppl1-27-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e0e/5448321/acbba0c2a57c/Cell-J-19-Suppl1-27-g06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e0e/5448321/6a881d4ce935/Cell-J-19-Suppl1-27-g07.jpg

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本文引用的文献

1
Updated Diagnostic Criteria and Staging System for Multiple Myeloma.多发性骨髓瘤的更新诊断标准和分期系统。
Am Soc Clin Oncol Educ Book. 2016;35:e418-23. doi: 10.1200/EDBK_159009.
2
Erratum to: MicroRNAs involved in the browning process of adipocytes.《脂肪细胞褐变过程中涉及的微小RNA》勘误
J Physiol Biochem. 2016 Sep;72(3):523-524. doi: 10.1007/s13105-016-0475-7.
3
PPARα induces cell apoptosis by destructing Bcl2.过氧化物酶体增殖物激活受体α（PPARα）通过破坏Bcl2诱导细胞凋亡。
鉴定异种移植瘤及其上升的成球细胞系属于EB病毒诱导的淋巴瘤，并对成球细胞的状态进行表征。
Cancer Cell Int. 2019 May 6;19:120. doi: 10.1186/s12935-019-0842-x. eCollection 2019.
Oncotarget. 2015 Dec 29;6(42):44635-42. doi: 10.18632/oncotarget.5988.
4
miR-204-5p promotes the adipogenic differentiation of human adipose-derived mesenchymal stem cells by modulating DVL3 expression and suppressing Wnt/β-catenin signaling.微小RNA-204-5p通过调节DVL3表达和抑制Wnt/β-连环蛋白信号传导来促进人脂肪来源间充质干细胞的成脂分化。
Int J Mol Med. 2015 Jun;35(6):1587-95. doi: 10.3892/ijmm.2015.2160. Epub 2015 Mar 31.
5
Multiple myeloma.多发性骨髓瘤
PET Clin. 2015 Apr;10(2):227-41. doi: 10.1016/j.cpet.2014.12.008. Epub 2015 Jan 24.
6
MicroRNA-204 Targets Runx2 to Attenuate BMP-2-induced Osteoblast Differentiation of Human Aortic Valve Interstitial Cells.微小RNA-204靶向Runx2以减弱骨形态发生蛋白-2诱导的人主动脉瓣间质细胞成骨分化。
J Cardiovasc Pharmacol. 2015 Jul;66(1):63-71. doi: 10.1097/FJC.0000000000000244.
7
Dkk-1 and IL-7 in plasma of patients with multiple myeloma prevent differentiation of mesenchymal stem cells into osteoblasts.多发性骨髓瘤患者血浆中的Dkk-1和IL-7可阻止间充质干细胞分化为成骨细胞。
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8
[Growth inhibition of human hepatocellular carcinoma by miRNA-204 via down-regulation of Bcl-2 and Sirt1 expression].[微小RNA-204通过下调Bcl-2和Sirt1表达抑制人肝癌细胞生长]
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2015 Feb;31(2):168-72.
9
Transformer 2β and miR-204 regulate apoptosis through competitive binding to 3' UTR of BCL2 mRNA.转录因子2β和微小RNA-204通过与BCL2信使核糖核酸的3'非翻译区竞争性结合来调节细胞凋亡。
Cell Death Differ. 2015 May;22(5):815-25. doi: 10.1038/cdd.2014.176. Epub 2014 Oct 24.
10
The role of microRNAs in cell fate determination of mesenchymal stem cells: balancing adipogenesis and osteogenesis.微小RNA在间充质干细胞细胞命运决定中的作用：平衡脂肪生成和成骨作用。
BMB Rep. 2015 Jun;48(6):319-23. doi: 10.5483/bmbrep.2015.48.6.206.