Key Laboratory of Colloid and Interface Chemistry & Key Laboratory of Special Aggregated Materials, Shandong University, Ministry of Education, Jinan, 250100, P.R. China), Fax: (+86)531-8856-4750.
State Key Laboratory of Solid Lubrication, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, 730000, P.R. China.
Chemistry. 2017 Aug 1;23(43):10413-10422. doi: 10.1002/chem.201701709. Epub 2017 Jul 10.
Self-assembly exploits noncovalent interactions to offer a facile and effective method for the construction of soft materials with multifunctionalities and diversity. In this work, fluorescence carbon quantum dots coordinated by Ce ions (CQDCe) have been synthesized and exploited as building blocks to generate a series of hierarchical structures through the ionic self-assembly of CQDCe and biomolecules, namely DNA, myoglobin (Mb), and hyaluronic acid (HA). In particular, vesicles can be constructed by the simple mixing of oppositely charged CQDCe and DNA in water. The formation of unusual vesicles can be explained by the self-assembly of CQDCe with a rearranged structure and the rigid DNA biomolecular scaffolds. This facile noncovalent self-assembly method has inspired the innovative use of virgin DNA as a building block to construct vesicles rather than resorting to a sophisticated synthesis. The self-assembly of CQDCe-biopolymers was accompanied by aggregation-induced photoluminescence (PL) quenching. The biosensing platform was designed to detect polypeptides and deoxyribonuclease I through competitive binding of CQDCe and enzymatic hydrolysis of the DNA backbone, respectively. We believe that the integrative self-assembly of CQDCe and DNA will enrich the theoretical study of vesicle formation by DNA molecules and extend the application of fluorescence carbon quantum dots in the biological field.
自组装利用非共价相互作用，为构建具有多功能和多样性的软材料提供了一种简便有效的方法。在这项工作中，我们合成了由铈离子配位的荧光碳量子点（CQDCe），并将其用作构建块，通过 CQDCe 与生物分子（即 DNA、肌红蛋白（Mb）和透明质酸（HA））的离子自组装，生成一系列分级结构。特别是，通过在水中简单混合带相反电荷的 CQDCe 和 DNA，就可以构建出囊泡。通过 CQDCe 与具有重排结构的刚性 DNA 生物分子支架的自组装，可以解释这种不寻常囊泡的形成。这种简便的非共价自组装方法激发了人们创新性地使用原始 DNA 作为构建囊泡的构建块，而不是依赖复杂的合成方法。CQDCe-生物聚合物的自组装伴随着聚集诱导的光致发光（PL）猝灭。该生物传感平台设计用于通过 CQDCe 的竞争结合和 DNA 骨架的酶解分别检测多肽和脱氧核糖核酸酶 I。我们相信，CQDCe 和 DNA 的综合自组装将丰富 DNA 分子囊泡形成的理论研究，并扩展荧光碳量子点在生物领域的应用。
