Gonzalez S M, da Silva C B, Lindquist A G, Bufalo I, Morotti F, Lisboa L A, Seneda M
Laboratory of Animal Reproduction, University of Londrina, Parana, Brazil.
Reprod Domest Anim. 2017 Oct;52(5):836-841. doi: 10.1111/rda.12986. Epub 2017 Jun 4.
The goal of this study was to determine the distribution of pre-antral follicles in the ovarian parenchyma of mares. For Experiment 1, each ovary was cut longitudinally at the greater curvature, performing two hemiovaries. After that, six fragments from each hemiovary were obtained, resulting in 12 fragments, which were divided into the innermost region of the parenchyma, the middle region and the outermost region. All the three obtained sections were cut transversally to obtain two fragments from each one. For Experiment 2, each ovary also submitted to a longitudinal cut on the greater curvature, forming two hemiovaries. Each hemiovary was sectioned into four symmetrical fragments, resulting in eight fragments per ovary. The fragments were related as being near to or far from the ovulatory fossa. The fragments of both experiments were immediately fixed in Carnoy for 12 hr and kept in 70% ethanol for 24 hr. Follicles were classified according to the stages of development and for morphological integrity according to oocyte morphology and granulosa cells. After the histological assessment, a total of 1,130 follicles were visualized from Experiment 1, being 1,054 (93.3%) primordial follicles and 76 (4.7%) follicles in development. The innermost region had the highest percentage of pre-antral follicles compared to the other regions (p < .05). The middle and outermost regions showed higher percentages of intact primordial and developing follicles than the innermost region (p < .05). Considering Experiment 2, 938 follicles were found, being 894 (95.3%) primordial and 44 (4.7%) follicles in development. The region near the ovulatory fossa presented higher (58.7%; 551 of 938) follicular concentration compared to the region far from the ovulatory fossa (41.3%; 387 of 938; p < .05). As a conclusion, distribution of pre-antral follicles in the equine ovary has a specific pattern through the parenchyma. Also, the follicular integrity differed in the studied ovarian areas.
本研究的目的是确定母马卵巢实质中窦前卵泡的分布情况。实验1中，每个卵巢在大弯处纵向切开，得到两个半卵巢。之后，从每个半卵巢获取6个片段，共得到12个片段，将其分为实质的最内层区域、中间区域和最外层区域。对这三个得到的切片都进行横向切割，每个切片再得到两个片段。实验2中，每个卵巢同样在大弯处进行纵向切割，形成两个半卵巢。每个半卵巢被切成四个对称的片段，每个卵巢共得到8个片段。这些片段被分为靠近或远离排卵窝。两个实验的片段均立即用卡诺氏固定液固定12小时，然后保存在70%乙醇中24小时。卵泡根据发育阶段进行分类，并根据卵母细胞形态和颗粒细胞评估形态完整性。组织学评估后，实验1共观察到1130个卵泡，其中1054个（93.3%）为原始卵泡，76个（4.7%）为发育中的卵泡。与其他区域相比，最内层区域的窦前卵泡百分比最高（p < 0.05）。中间区域和最外层区域完整的原始卵泡和发育中卵泡的百分比高于最内层区域（p < 0.05）。在实验2中，共发现938个卵泡，其中894个（95.3%）为原始卵泡，44个（4.7%）为发育中的卵泡。与远离排卵窝的区域（41.3%；938个中的387个）相比，靠近排卵窝的区域卵泡浓度更高（58.7%；938个中的551个；p < 0.05）。总之，母马卵巢中窦前卵泡在实质中的分布具有特定模式。此外，在所研究的卵巢区域中卵泡的完整性也有所不同。
