Cha Ho-Yeol, Ahn Sang-Hyun, Cheon Jin-Hong, Park Sun-Young, Kim Kibong
Department of Korean Pediatrics, Hospital of Korean Medicine, Pusan National University, Geumo-ro 20, Mulgeum-eup, Yangsan-si, Gyeongsangnam-do 50612, Republic of Korea; Department of Korean Pediatrics, School of Korean Medicine, Pusan National University, Pusandaehak-ro 49, Mulgeum-eup, Yangsan-si, Gyeongsangnam-do 50612, Republic of Korea.
Department of Anatomy, College of Korean Medicine, Semyung University, Semyung-ro 65, Jecheon-si, Chungbuk 27136, Republic of Korea.
J Ethnopharmacol. 2017 Jul 12;206:327-336. doi: 10.1016/j.jep.2017.06.001. Epub 2017 Jun 3.
Hataedock treatment is traditionally used for the purpose of preventing the future skin disease by feeding herbal extracts to the newborn in traditional Chinese and Korean medicine.
This study investigated the preventive therapeutic effects of Hataedock (HTD) treatment for atopic dermatitis (AD) through skin barrier protection in Dermatophagoides farinae-induced NC/Nga mice.
To the HTD treatment group, the extract of Coptis japonica Makino and Glycyrrhiza uralensis Fischer, which analyzed with High Performance Liquid Chromatography (HPLC)-fingerprint for quality consistency, was administered orally to the 3-week-old mice before inducing AD. After that, Dermatophagoides farinae was applied except the control group to induce AD-like skin lesions. We confirmed the effects of HTD on morphological changes, protection of skin barrier, regulation of Th2 differentiation, inflammation regulation and induction of apoptosis through histochemistry, immunohistochemistry, and Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay.
HTD effectively reduced edema, angiogenesis and skin lesion. HTD also increased the levels of liver X receptor (LXR) and filaggrin but decreased the level of protein kinase C (PKC) (p<0.01). The levels of interleukin-4 (IL-4), IL-13, signal transducer and activator of transcription-6 (STAT-6) and Cluster of differentiation 40 (CD40) were significantly reduced in the HTD treated group (p<0.01). HTD also suppressed the mast cell degranulation and the level of the high-affinity IgE receptor (FcɛRI), substance P, Matrix metalloproteinases-9 (MMP-9) and 5-hydroxytryptamine (5-HT) (p<0.01). The levels of inflammatory factors such as nuclear factor-kappaB (NF-κB) p65, phosphorylated IκB (p-IκB) and inducible nitric oxide synthase (iNOS) were also decreased (p<0.01). Apoptosis of inflammatory cells was also found to increase (p<0.01).
Our results indicate that HTD effectively regulate the Th2 differentiation, mast cell activation and various inflammatory responses on AD-induced mice through protection of skin barrier. Therefore, HTD may have potential applications for alternative and preventive treatment in the management of AD.
在传统中医和韩医学中，通过给新生儿喂食草药提取物，传统上使用孩儿参治疗来预防未来的皮肤病。
本研究通过在粉尘螨诱导的NC/Nga小鼠中进行皮肤屏障保护，研究孩儿参（HTD）治疗对特应性皮炎（AD）的预防治疗作用。
在诱导AD之前，将用高效液相色谱（HPLC）指纹图谱分析质量一致性的黄连提取物和甘草提取物口服给予3周龄小鼠，作为HTD治疗组。之后，除对照组外，应用粉尘螨诱导AD样皮肤病变。我们通过组织化学、免疫组织化学和末端脱氧核苷酸转移酶dUTP缺口末端标记（TUNEL）试验，证实了HTD对形态学变化、皮肤屏障保护、Th2分化调节、炎症调节和细胞凋亡诱导的影响。
HTD有效减轻水肿、血管生成和皮肤病变。HTD还增加了肝脏X受体（LXR）和丝聚合蛋白的水平，但降低了蛋白激酶C（PKC）的水平（p<0.01）。在HTD治疗组中，白细胞介素-4（IL-4）、IL-13、信号转导和转录激活因子-6（STAT-6）以及分化簇40（CD40）的水平显著降低（p<0.01）。HTD还抑制肥大细胞脱颗粒以及高亲和力IgE受体（FcɛRI）、P物质、基质金属蛋白酶-9（MMP-9）和5-羟色胺（5-HT）的水平（p<0.01）。核因子-κB（NF-κB）p65、磷酸化IκB（p-IκB）和诱导型一氧化氮合酶（iNOS）等炎症因子的水平也降低（p<0.01）。还发现炎症细胞凋亡增加（p<0.01）。
我们的结果表明，HTD通过保护皮肤屏障有效调节AD诱导小鼠的Th2分化、肥大细胞活化和各种炎症反应。因此，HTD在AD的管理中可能具有替代和预防性治疗的潜在应用。
