Federal Research Center Institute of Cytology and Genetics, the Siberian Branch of the Russian Academy of Sciences, 10 Lavrentyeva Ave., Novosibirsk, 630090, Russia.
Siberian Federal Biomedical Research Center, Ministry of Healthcare of the Russian Federation, 15 Rechkunovskaya St., Novosibirsk, 630055, Russia.
Sci Rep. 2017 Jun 5;7(1):2787. doi: 10.1038/s41598-017-02632-0.
MicroRNAs (miRNAs) constitute a class of small noncoding RNAs that plays an important role in the post-transcriptional regulation of gene expression. Much evidence has demonstrated that miRNAs are involved in regulating the human and mouse pluripotency. Nevertheless, to our knowledge, miRNAs in the pluripotent stem cells of one of the most commonly used model organisms - the Rattus norvegicus have not been studied. In the present study, we performed deep sequencing of small RNA molecules in the embryonic fibroblasts, embryonic stem cells, and induced pluripotent stem cells of laboratory rats. Bioinformatics analysis revealed 674 known miRNAs and 394 novel miRNA candidates in all of the samples. Expression of known pluripotency-associated miRNAs, such as the miR-290-295 and miR-183-96-182 clusters as well as members of the miR-200 family, was detected in rat pluripotent stem cells. Analysis of the targets of differentially expressed known and novel miRNAs showed their involvement in the regulation of pluripotency and the reprogramming process in rats. Bioinformatics and systems biology approaches identified potential pathways that are regulated by these miRNAs. This study contributes to our understanding of miRNAs in the regulation of pluripotency and cell reprogramming in the laboratory rat.
微小 RNA（miRNA）是一类小的非编码 RNA，在基因表达的转录后调控中发挥着重要作用。大量证据表明，miRNA 参与调节人类和小鼠的多能性。然而，据我们所知，在最常用的模式生物之一——褐家鼠的多能干细胞中，miRNA 尚未得到研究。在本研究中，我们对实验室大鼠的胚胎成纤维细胞、胚胎干细胞和诱导多能干细胞中的小分子 RNA 进行了深度测序。生物信息学分析揭示了所有样本中 674 个已知 miRNA 和 394 个新的 miRNA 候选物。在大鼠多能干细胞中检测到了已知与多能性相关的 miRNA 的表达，如 miR-290-295 和 miR-183-96-182 簇以及 miR-200 家族的成员。差异表达的已知和新 miRNA 的靶标分析表明它们参与了大鼠多能性和重编程过程的调控。生物信息学和系统生物学方法鉴定了这些 miRNA 调控的潜在途径。本研究有助于我们理解 miRNA 在实验室大鼠多能性和细胞重编程中的调控作用。
