Academy of Scientific and Innovative Research (AcSIR), CSIR-National Institute of Oceanography, Dona-Paula, 403004, Goa, India.
Biological Oceanography Division, CSIR-National Institute of Oceanography, Dona-Paula, Goa, 403004, India.
Appl Biochem Biotechnol. 2018 Jan;184(1):35-47. doi: 10.1007/s12010-017-2523-3. Epub 2017 Jun 6.
Increased awareness regarding the harmful effects of ultraviolet (UV)-B radiation has led to the search for new sources of natural UV-B protecting compounds. Mycosporine-like amino acids are one of such promising compounds found in several organisms. Cyanobacteria are ideal organisms for isolation of these compounds due to their compatibility and adaptability to thrive under harsh environmental conditions. In the following investigation, we report the production of shinorine in Leptolyngbya sp. isolated from the intertidal region. Based on the spectral characteristics and liquid chromatography-mass spectrometry analysis, the UV-absorbing compound was identified as shinorine. To the best of our knowledge, this is the first report on the occurrence of shinorine in Leptolyngbya sp. We also investigated the effect of artificial UV-B radiation and periodic desiccation on chlorophyll-a, total carotenoids, and mycosporine-like amino acids (MAAs) production. The UV-B radiation had a negative effect on growth and chlorophyll concentration, whereas it showed an inductive effect on the production of total carotenoids and MAAs. Desiccation along with UV-B radiation led to an increase in the concentration of photoprotective compounds. These results indicate that carotenoids and MAAs thus facilitate cyanobacteria to avoid and protect themselves from the deleterious effects of UV-B and desiccation.
人们对紫外线（UV）-B 辐射的有害影响的认识不断提高，促使人们寻找新的天然 UV-B 保护化合物来源。 菌 氨酸类似物是在几种生物体中发现的一种有前途的化合物。 由于其相容性和适应在恶劣环境条件下茁壮成长的能力，蓝藻是分离这些化合物的理想生物。 在以下研究中，我们报告了从潮间带分离出的 Leptolyngbya sp. 中 shinorine 的生产。 根据光谱特征和液相色谱-质谱分析，鉴定出具有紫外吸收的化合物为 shinorine。 据我们所知，这是 shinorine 在 Leptolyngbya sp. 中存在的首次报道。 我们还研究了人工 UV-B 辐射和周期性干燥对叶绿素 a、总类胡萝卜素和菌 氨酸类似物（MAAs）生产的影响。UV-B 辐射对生长和叶绿素浓度有负面影响，但对总类胡萝卜素和 MAAs 的产生有诱导作用。 干燥与 UV-B 辐射一起导致光保护化合物浓度增加。 这些结果表明，类胡萝卜素和 MAAs 有助于蓝藻避免和保护自己免受 UV-B 和干燥的有害影响。
