用巴西副球孢子菌的辐射减毒酵母细胞对小鼠进行免疫接种。

Mouse Immunization with Radioattenuated Yeast Cells of Paracoccidioides brasiliensis.

作者信息

do Nascimento Martins Estefânia Mara, de Andrade Antero Silva Ribeiro

机构信息

Centro de Desenvolvimento da Tecnologia Nuclear (CDTN), Rua Professor Mário Werneck S/N°, Cidade Universitária-Campus da UFMG, 31120-970, Belo Horizonte, MG, Brazil.

出版信息

Methods Mol Biol. 2017;1625:3-21. doi: 10.1007/978-1-4939-7104-6_1.

DOI:10.1007/978-1-4939-7104-6_1

PMID:28584979

Abstract

Paracoccidioides brasiliensis is the agent of paracoccidioidomycosis, the most prevalent mycosis in Latin America, and currently there is no effective vaccine. The present chapter describes the methodology to obtain radioattenuated yeast cells of P. brasiliensis and a protocol to evaluate protective response elicited by this immunogen in experimental paracoccidioidomycosis. The radioattenuated yeast provides a valuable tool for immunological studies in experimental paracoccidioidomycosis and vaccine research.

摘要

巴西副球孢子菌是拉丁美洲最常见的真菌病——副球孢子菌病的病原体，目前尚无有效的疫苗。本章介绍了获得巴西副球孢子菌辐射减毒酵母细胞的方法，以及评估这种免疫原在实验性副球孢子菌病中引发的保护性反应的方案。辐射减毒酵母为实验性副球孢子菌病的免疫学研究和疫苗研究提供了一个有价值的工具。