Sato Toshitsugu, Irie Toshikazu, Yoshino Fumihiko
a Iwate Biotechnology Research Center , Kitakami-shi , Japan.
b Department of Biotechnology and Environmental Chemistry , National University Corporation Kitami Institute of Technology , Kitami , Japan.
Biosci Biotechnol Biochem. 2017 Aug;81(8):1553-1556. doi: 10.1080/09168451.2017.1332977. Epub 2017 Jun 6.
Lentinula edodes (shiitake), which have a powerful ligninolytic system, is one of the most important edible mushrooms in Asia. In this study, we introduced the manganese peroxidase (MnP, EC 1.11.1.13) gene from Pleurotus ostreatus driven by L. edodes laccase 1 gene promoter into L. edodes for expression. The resulting transformant expressed the recombinant gene and showed a higher level of MnP activity than that of the wild-type strain.
香菇具有强大的木质素分解系统，是亚洲最重要的食用蘑菇之一。在本研究中，我们将由香菇漆酶1基因启动子驱动的来自糙皮侧耳的锰过氧化物酶（MnP，EC 1.11.1.13）基因导入香菇进行表达。所得转化体表达了重组基因，并且显示出比野生型菌株更高水平的MnP活性。
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