白腐真菌平菇转化体过量产生细胞内cAMP和漆酶。
The white-rot fungus pleurotus ostreatus transformant overproduced intracellular cAMP and laccase.
作者信息
Yao Yuki, Sakamoto Takaiku, Honda Yoichi, Kagotani Yasuyuki, Izumitsu Kousuke, Suzuki Kazumi, Irie Toshikazu
机构信息
Environmental Science Graduate School, The University of Shiga Prefecture.
出版信息
Biosci Biotechnol Biochem. 2013;77(11):2309-11. doi: 10.1271/bbb.130470. Epub 2013 Nov 7.
Transformation of Pleurotus ostreatus PC9 with the mutated heterotrimeric G protein alpha subunit (Gα) gene resulted in higher laccase (Lac) activity and intracellular cyclic adenosine monophosphate (cAMP) concentrations as compared to those in wild-type PC9. The transformant also exhibited higher Lac activity than the wild type when cultured in a medium containing known Lac inducers CuSO4 and ferulic acid.
用突变的异源三聚体G蛋白α亚基（Gα）基因转化糙皮侧耳PC9，与野生型PC9相比，导致漆酶（Lac）活性和细胞内环状单磷酸腺苷（cAMP）浓度更高。当在含有已知Lac诱导剂硫酸铜和阿魏酸的培养基中培养时，该转化体也表现出比野生型更高的Lac活性。
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