Department of Biomedical Engineering, Stony Brook University, Stony Brook, NY 11794-5281, USA.
Lab Chip. 2017 Jun 27;17(13):2186-2192. doi: 10.1039/c7lc00283a.
We present a novel method for conducting true single-cell encapsulation at very high efficiency for the manipulation of precious samples. Our unique strategy is based on the sequential capture and original encapsulation of single-cells into a series of hydrodynamic traps. We identified two distinct modes of encapsulation and we established their associated design rules. We improved the trapping scheme to reach a near perfect capture efficiency and make it compatible with the encapsulation process. Finally, we developed the complete device operation that permits highly efficient single-cell encapsulation and droplet retrieval. This platform provides the foundation to a fully integrated multiparameter platform that will impact the analysis of tissues at single-cell resolution.
我们提出了一种新的方法,可以非常高效地进行真正的单细胞包封,以处理珍贵的样本。我们独特的策略基于单细胞的顺序捕获和原始包封到一系列流体动力阱中。我们确定了两种不同的包封模式,并建立了它们相关的设计规则。我们改进了捕获方案,以达到近乎完美的捕获效率,并使其与包封过程兼容。最后,我们开发了完整的设备操作,允许高效的单细胞包封和液滴检索。该平台为完全集成的多参数平台提供了基础,将影响单细胞分辨率下组织的分析。