Cengiz Ibrahim Fatih, Pereira Hélder, Pêgo José Miguel, Sousa Nuno, Espregueira-Mendes João, Oliveira Joaquim Miguel, Reis Rui Luís
3Bs Research Group, Biomaterials, Biodegradables and Biomimetics, University of Minho, Barco GMR, Portugal.
ICVS/3Bs, PT Government Associated Laboratory, Guimarães-Braga, Portugal.
J Tissue Eng Regen Med. 2017 Jun;11(6):1844-1852. doi: 10.1002/term.2082. Epub 2015 Sep 7.
The knee menisci have important roles in the knee joint. Complete healing of the meniscus remains a challenge in the clinics. Cellularity is one of the most important biological parameters that must be taken into account in regenerative strategies. However, knowledge on the 3D cellularity of the human meniscus is lacking in the literature. The aim of this study was to quantify the 3D cellular density of human meniscus from the osteoarthritic knee in a segmental and regional manner with respect to laterality. Human lateral menisci were histologically processed and stained with Giemsa for histomorphometric analysis. The cells were counted in an in-depth fashion. 3D cellular density in the vascular region (27 199 cells/mm ) was significantly higher than in the avascular region (12 820 cells/mm ). The cells were observed to possess two distinct morphologies, roundish or flattened. The 3D density of cells with fibrochondrocyte morphology (14 705 cells/mm ) was significantly greater than the 3D density of the cells with fibroblast-like cell morphology (5539 cells/mm ). The best-fit equation for prediction of the 3D density of cells with fibrochondrocyte morphology was found to be: Density of cells with fibrochondrocyte morphology = 1.22 × density of cells withfibroblast-like cell morphology + 7750. The present study revealed the segmental and regional 3D cellular density of human lateral meniscus from osteoarthritic knee with respect to laterality. This crucial but so far missing information will empower cellular strategies aiming at meniscus tissue regeneration. Copyright © 2015 John Wiley & Sons, Ltd.
膝关节半月板在膝关节中发挥着重要作用。半月板的完全愈合在临床上仍然是一项挑战。细胞密度是再生策略中必须考虑的最重要生物学参数之一。然而，文献中缺乏关于人类半月板三维细胞密度的相关知识。本研究的目的是按侧别以节段性和区域性方式量化骨关节炎膝关节中人类半月板的三维细胞密度。对人类外侧半月板进行组织学处理，并用吉姆萨染色进行组织形态计量学分析。对细胞进行深入计数。血管区域的三维细胞密度（27199个细胞/mm³）显著高于无血管区域（12820个细胞/mm³）。观察到细胞具有两种不同的形态，圆形或扁平形。具有纤维软骨细胞形态的细胞的三维密度（14705个细胞/mm³）显著大于具有成纤维细胞样细胞形态的细胞的三维密度（5539个细胞/mm³）。发现预测具有纤维软骨细胞形态的细胞三维密度的最佳拟合方程为：具有纤维软骨细胞形态的细胞密度 = 1.22×具有成纤维细胞样细胞形态的细胞密度 + 7750。本研究揭示了骨关节炎膝关节人类外侧半月板按侧别的节段性和区域性三维细胞密度。这一关键但迄今缺失信息将有助于针对半月板组织再生的细胞策略。版权所有©2015约翰威立父子有限公司。
