Van Holle Sofie, De Schutter Kristof, Eggermont Lore, Tsaneva Mariya, Dang Liuyi, Van Damme Els J M
Department of Molecular Biotechnology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000 Ghent, Belgium.
Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000 Ghent, Belgium.
Int J Mol Sci. 2017 May 25;18(6):1136. doi: 10.3390/ijms18061136.
Lectins are present throughout the plant kingdom and are reported to be involved in diverse biological processes. In this study, we provide a comparative analysis of the lectin families from model species in a phylogenetic framework. The analysis focuses on the different plant lectin domains identified in five representative core angiosperm genomes (, , , ssp. and ssp. ). The genomes were screened for genes encoding lectin domains using a combination of Basic Local Alignment Search Tool (BLAST), hidden Markov models, and InterProScan analysis. Additionally, phylogenetic relationships were investigated by constructing maximum likelihood phylogenetic trees. The results demonstrate that the majority of the lectin families are present in each of the species under study. Domain organization analysis showed that most identified proteins are multi-domain proteins, owing to the modular rearrangement of protein domains during evolution. Most of these multi-domain proteins are widespread, while others display a lineage-specific distribution. Furthermore, the phylogenetic analyses reveal that some lectin families evolved to be similar to the phylogeny of the plant species, while others share a closer evolutionary history based on the corresponding protein domain architecture. Our results yield insights into the evolutionary relationships and functional divergence of plant lectins.
凝集素存在于整个植物界，据报道参与多种生物过程。在本研究中，我们在系统发育框架下对模式物种的凝集素家族进行了比较分析。该分析聚焦于在五个有代表性的核心被子植物基因组（、、、亚种和亚种）中鉴定出的不同植物凝集素结构域。使用基本局部比对搜索工具（BLAST）、隐马尔可夫模型和InterProScan分析相结合的方法，对这些基因组进行编码凝集素结构域的基因筛选。此外，通过构建最大似然系统发育树来研究系统发育关系。结果表明，大多数凝集素家族存在于所研究的每个物种中。结构域组织分析表明，由于进化过程中蛋白质结构域的模块化重排，大多数鉴定出的蛋白质是多结构域蛋白。这些多结构域蛋白中的大多数广泛分布，而其他一些则表现出谱系特异性分布。此外，系统发育分析表明，一些凝集素家族的进化与植物物种的系统发育相似，而另一些则基于相应的蛋白质结构域结构具有更密切的进化历史。我们的结果为植物凝集素的进化关系和功能分化提供了见解。
