Han Bing, Bu Peili, Meng Xiao, Hou Xiaoyang
Internal Medicine-Cardiovascular Department, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China.
Internal Medicine-Cardiovascular Department, Shandong Province-owned Hospital, Jinan, Shandong 250012, P.R. China.
Exp Ther Med. 2017 Jun;13(6):2657-2666. doi: 10.3892/etm.2017.4355. Epub 2017 Apr 18.
Idiopathic pulmonary arterial hypertension (IPAH) is a fatal disease with a poor prognosis and the molecular pathways underlying the pathogenesis of IPAH are not fully understood. In the present study, the long non-coding RNA (lncRNA) and mRNA expression profiles of lymphocytes obtained from 12 IPAH patients and 12 healthy controls were analyzed using Arraystar Human lncRNA Microarray v2.0, and their roles in the pathogenesis of IPAH were characterized using comprehensive bioinformatic tools. A total of 2,511 lncRNAs (2,004 upregulated and 507 downregulated) and 1,169 mRNAs (609 upregulated and 560 downregulated) were aberrantly expressed in IPAH patients with a fold-change of >2.0. Gene ontology analysis indicated that the coexpressed lncRNAs and mRNAs were involved in the process of translation, while pathway analysis indicated that the coexpressed RNAs were enriched during the process of oxidative phosphorylation and in the ribosome. It was concluded that dysregulated lncRNAs are potentially associated with IPAH, and aberrant lncRNA expression in blood cells may serve as a diagnostic marker of IPAH.
特发性肺动脉高压（IPAH）是一种预后较差的致命性疾病，其发病机制的分子途径尚未完全明确。在本研究中，使用Arraystar Human lncRNA Microarray v2.0分析了12例IPAH患者和12例健康对照者淋巴细胞的长链非编码RNA（lncRNA）和mRNA表达谱，并使用综合生物信息学工具对它们在IPAH发病机制中的作用进行了表征。在IPAH患者中，共有2511个lncRNA（2004个上调，507个下调）和1169个mRNA（609个上调，560个下调）异常表达，变化倍数>2.0。基因本体分析表明，共表达的lncRNA和mRNA参与翻译过程，而通路分析表明，共表达的RNA在氧化磷酸化过程和核糖体中富集。得出的结论是，lncRNA失调可能与IPAH相关，血细胞中lncRNA异常表达可能作为IPAH的诊断标志物。
