Zeng Lin-Ru, Zhu Fang-Bing, Wang Jian-Yue, Hou Qiao, Yue Zhen-Shuang, Yan Shi-Gui, Quan Ren-Fu, Zhang Ying-Liang
Department of Orthopaedic Surgery, Xiaoshan Traditional Chinese Medical Hospital, Hangzhou, Zhejiang 311200, P.R. China.
Department of Orthopaedic Surgery, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310009, P.R. China.
Exp Ther Med. 2017 Jun;13(6):2934-2938. doi: 10.3892/etm.2017.4327. Epub 2017 Apr 12.
We studied the effect of molecular polyethylene particles on local heterotopic ossification. A total of 36 healthy Sprague-Dawley rats were randomly divided into the control group (n=18) and the observation group (n=18). High molecular polyethylene particles were injected to rupture Achilles tendon position in the observation group, and normal saline was injected in the control group. X-ray examinations were conducted on Achilles tendon in the 4th, 8th and 12th week after operation. The incidence rate of heterotopic ossification was evaluated, and bone trabecula morphological structure was studied under optical microscope after hematoxylin and eosin staining. Bone morphogenetic protein 2 (BMP-2), transforming growth factor-β (TGF-β), interleukin-1 (IL-1), tumor necrosis factor-α (TNF-α), runt-related transcription factor 2 (Runx2) and matrix metalloproteinase-9 (MMP-9) expression levels were also measured. Our results showed that heterotopic ossification incidence in the observation group was significantly lower than that in the control group. Achilles tendon structure in the control group increased in volume, and its texture was harder and cartilage-like. In the observation group, trabecular bone volume, thickness and quantity were more than those observed in the control group. BMP-2, TGF-β, IL-1, TNF-α, Runx2 and MMP-9 levels in the observation group were significantly lower than those in the control group. We concluded that, high molecular polyethylene particles had a significant inhibiting effect on local heterotopic ossification.
我们研究了分子态聚乙烯颗粒对局部异位骨化的影响。总共36只健康的Sprague-Dawley大鼠被随机分为对照组（n = 18）和观察组（n = 18）。在观察组中，将高分子聚乙烯颗粒注射到跟腱断裂部位，而在对照组中注射生理盐水。在术后第4、8和12周对跟腱进行X线检查。评估异位骨化的发生率，并在苏木精和伊红染色后在光学显微镜下研究骨小梁的形态结构。还测量了骨形态发生蛋白2（BMP-2）、转化生长因子-β（TGF-β）、白细胞介素-1（IL-1）、肿瘤坏死因子-α（TNF-α）、 runt相关转录因子2（Runx2）和基质金属蛋白酶-9（MMP-9）的表达水平。我们的结果表明，观察组的异位骨化发生率明显低于对照组。对照组的跟腱结构体积增大，质地更硬且类似软骨。在观察组中，骨小梁体积、厚度和数量均多于对照组。观察组中BMP-2、TGF-β、IL-1、TNF-α、Runx2和MMP-9的水平明显低于对照组。我们得出结论，高分子聚乙烯颗粒对局部异位骨化有显著的抑制作用。
