Webb S M, Lewiński A K, Steger R W, Reiter R J, Bartke A
Life Sci. 1985 Apr 1;36(13):1239-45. doi: 10.1016/0024-3205(85)90268-1.
Snell dwarf mice (dw/dw) are characterized by a genetically determined, congenital lack of pituitary GH, TSH and prolactin. Given that hypothalamic somatostatin is involved in the regulation of pituitary GH and TSH release, it was decided to investigate the content of immunoreactive somatostatin (IRS) in the median eminence of dw/dw and phenotypically normal mice of the same strain. The content of IRS in the pyloric antrum and pineal gland of these animals was also examined. The effects of ovariectomy and of hyperprolactinemia (induced by a pituitary graft under the kidney capsule) on the median eminence content of IRS were also studied in both normal and dwarf mice. Median eminence IRS content was significantly lower in the dw/dw (23.6 +/- 1.8 ng) than in normal mice (57.4 +/- 7.1 ng); no difference was found in the pyloric IRS content of dw/dw (16.9 +/- 1.6 ng/mg of protein) and normal animals (13.8 +/- 1.9 ng/mg of protein), nor in the pineal content of IRS (639.4 +/- 64.4 pg/gland in the dw/dw; 732 +/- 265 pg/gland in normals). Neither ovariectomy nor hyperprolactinemia were found to affect the IRS content in the tissues studied in normal or dwarf mice. Treatment of an additional group of 9 dwarf mice with L-thyroxine (L-T4 2 micrograms/48 h. s.c. for 2 weeks) significantly increased the animals weight (10.2 +/- 0.4 g versus 7.4 +/- 0.3 g) and produced maturation of facial features; however, it did not change the IRS content in any of the tissues studied. It is concluded that the content of IRS in the median eminence of mice with a congenital lack of GH, TSH and prolactin is significantly reduced and that this is unlikely to be related to the deficiency of thyroid hormones in these animals.
斯内尔侏儒小鼠（dw/dw）的特征是由基因决定的先天性缺乏垂体生长激素、促甲状腺激素和催乳素。鉴于下丘脑生长抑素参与垂体生长激素和促甲状腺激素释放的调节，因此决定研究dw/dw小鼠和同一品系表型正常小鼠正中隆起中免疫反应性生长抑素（IRS）的含量。还检测了这些动物幽门窦和松果体中IRS的含量。在正常和侏儒小鼠中，还研究了卵巢切除术和高催乳素血症（通过肾包膜下垂体移植诱导）对正中隆起IRS含量的影响。dw/dw小鼠（23.6±1.8纳克）正中隆起IRS含量显著低于正常小鼠（57.4±7.1纳克）；dw/dw小鼠（16.9±1.6纳克/毫克蛋白质）和正常动物（13.8±1.9纳克/毫克蛋白质）的幽门IRS含量以及IRS的松果体含量均未发现差异（dw/dw小鼠为639.4±64.4皮克/腺体；正常小鼠为732±265皮克/腺体）。未发现卵巢切除术和高催乳素血症会影响正常或侏儒小鼠所研究组织中的IRS含量。用左旋甲状腺素（L-T4 2微克/48小时，皮下注射，共2周）治疗另一组9只侏儒小鼠，显著增加了动物体重（10.2±0.4克对7.4±0.3克）并使面部特征成熟；然而，它并未改变所研究任何组织中的IRS含量。得出的结论是，先天性缺乏生长激素、促甲状腺激素和催乳素的小鼠正中隆起中IRS的含量显著降低，并且这不太可能与这些动物甲状腺激素缺乏有关。
