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出芽短梗霉产生的β-葡聚糖可有效增强黑丸大豆提取物诱导的血栓调节蛋白-1表达。

Aureobasidium pullulans produced β-glucan is effective to enhance Kurosengoku soybean extract induced Thrombospondin-1 expression.

机构信息

Aureo Science Co., Ltd., Hokudai Business Spring, North 21, West 12, Kita-ku, Sapporo, Hokkaido, 001-0021, Japan.

Aureo Co., Ltd., 54-1 Kazusakoito, Kimitsu, Chiba, 292-1149, Japan.

出版信息

Sci Rep. 2017 Jun 6;7(1):2831. doi: 10.1038/s41598-017-03053-9.

DOI:10.1038/s41598-017-03053-9
PMID:28588201
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5460285/
Abstract

Black yeast, Aureobasidium pullulans is extracellularly produced β-(1,3), (1,6)-D-glucan (β-glucan) under certain conditions. In this study, using Glycine max cv. Kurosengoku (Kurosengoku soybeans), the production of β-glucan through fermentation of A. pullulans was evaluated, and the effects of A. pullulans cultured fluid (AP-CF) containing β-glucan made with Kurosengoku soybeans (kAP-CF) on a human monocyte derived cell line, Mono Mac 6 cells were investigated. Concentration of β-glucan in kAP-CF reached the same level as normal AP-CF. An anti-angiogenic protein, Thrombospondin-1 (THBS1) was effectively induced after the stimulation with kAP-CF for comparison with AP-CF. The THBS1 is also induced after stimulation with hot water extract of Kurosengoku soybeans (KS-E), while the combined stimulation of β-glucan with KS-E more effectively induced THBS1 than that with KS-E alone. These results suggest effects of A. pullulans-produced β-glucan on the enhancement of Kurosengoku soybean-induced THBS1 expression.

摘要

黑酵母，出芽短梗霉在某些条件下会在细胞外产生β-(1,3),(1,6)-D-葡聚糖（β-葡聚糖）。在这项研究中，利用大豆品种黑豆（Kurosengoku 大豆），通过出芽短梗霉发酵生产β-葡聚糖，并对含有 Kurosengoku 大豆β-葡聚糖的出芽短梗霉培养液（kAP-CF）对人单核细胞系 Mono Mac 6 细胞的影响进行了研究。kAP-CF 中的β-葡聚糖浓度达到了与正常 AP-CF 相同的水平。与 AP-CF 相比，kAP-CF 刺激后可有效诱导抗血管生成蛋白，即血小板反应蛋白-1（THBS1）。用黑豆热水提取物（KS-E）刺激后也会诱导 THBS1，但与单独用 KS-E 刺激相比，用β-葡聚糖和 KS-E 联合刺激更能有效诱导 THBS1。这些结果表明，出芽短梗霉产生的β-葡聚糖对增强黑豆诱导 THBS1 表达有一定作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/13b9/5460285/ef86dbf5a45d/41598_2017_3053_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/13b9/5460285/a9133c8c950f/41598_2017_3053_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/13b9/5460285/bcfe5d363abc/41598_2017_3053_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/13b9/5460285/f3f563247d15/41598_2017_3053_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/13b9/5460285/ef86dbf5a45d/41598_2017_3053_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/13b9/5460285/a9133c8c950f/41598_2017_3053_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/13b9/5460285/bcfe5d363abc/41598_2017_3053_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/13b9/5460285/f3f563247d15/41598_2017_3053_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/13b9/5460285/ef86dbf5a45d/41598_2017_3053_Fig4_HTML.jpg

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