Bernauer W
Naunyn Schmiedebergs Arch Pharmacol. 1985 Jan;328(3):288-94. doi: 10.1007/BF00515555.
The left coronary artery of rats was ligated either permanently, or for a period of 40 or 60 min, with subsequent reperfusion. In experiments with permanent occlusion, the hearts were removed and investigated 5 h after the coronary ligation, or immediately after death in animals which died earlier. The hearts from the reperfusion experiments were investigated 60 min after reopening the occluded artery. The extent of the ischaemic and necrotic areas of the hearts was determined. A quantitative photometric method was developed for this purpose, using "negative staining" with Evans blue for the ischaemic area, and "negative staining" with triphenyltetrazolium chloride for the necrotic area. In experiments in which ligation was permanent, the percentage of the ischaemic area which underwent necrosis increased with the time after coronary occlusion. In reperfusion experiments, myocardial necrosis was detected earlier than in experiments with permanent coronary ligation. The beta-adrenoceptor blocking agents pindolol, propranolol, and metoprolol significantly decreased the percentage of necrosis in experiments with permanent ligation of the coronary artery. The most selective of the beta-adrenoceptor blockers, i.e. metoprolol was tested in the reperfusion experiments. In these experiments, the amount of necrosis was also significantly decreased.
将大鼠的左冠状动脉永久性结扎，或结扎40或60分钟，随后再灌注。在永久性闭塞实验中，在冠状动脉结扎后5小时取出心脏进行研究，或在较早死亡的动物死亡后立即进行研究。再灌注实验的心脏在重新开放闭塞动脉60分钟后进行研究。测定心脏缺血和坏死区域的范围。为此开发了一种定量光度法，对缺血区域用伊文思蓝进行“负染色”，对坏死区域用氯化三苯基四氮唑进行“负染色”。在永久性结扎的实验中，发生坏死的缺血区域百分比随冠状动脉闭塞后的时间增加。在再灌注实验中，心肌坏死比永久性冠状动脉结扎实验中更早被检测到。β-肾上腺素能受体阻滞剂吲哚洛尔、普萘洛尔和美托洛尔在冠状动脉永久性结扎实验中显著降低坏死百分比。β-肾上腺素能受体阻滞剂中选择性最高的美托洛尔在再灌注实验中进行了测试。在这些实验中，坏死量也显著减少。
