Väänänen Tuija, Lehtimäki Lauri, Vuolteenaho Katriina, Hämäläinen Mari, Oksa Panu, Vierikko Tuula, Järvenpää Ritva, Uitti Jukka, Kankaanranta Hannu, Moilanen Eeva
The Immunopharmacology Research Group, Faculty of Medicine and Life Sciences, University of Tampere and Tampere University Hospital, P.O. Box 100, 33014 Tampere, Finland.
Allergy Centre, Tampere University Hospital and Faculty of Medicine and Life Sciences, University of Tampere, P.O. Box 100, 33014 Tampere, Finland.
Mediators Inflamm. 2017;2017:1797512. doi: 10.1155/2017/1797512. Epub 2017 May 14.
YKL-40 is a chitinase-like glycoprotein produced by alternatively activated macrophages that are associated with wound healing and fibrosis. Asbestosis is a chronic asbestos-induced lung disease, in which injury of epithelial cells and activation of alveolar macrophages lead to enhanced collagen production and fibrosis. We studied if YKL-40 is related to inflammation, fibrosis, and/or lung function in subjects exposed to asbestosis. Venous blood samples were collected from 85 men with moderate or heavy occupational asbestos exposure and from 28 healthy, age-matched controls. Levels of plasma YKL-40, CRP, IL-6, adipsin, and MMP-9 were measured with enzyme-linked immunosorbent assay (ELISA). Plasma YKL-40 levels were significantly higher in subjects with asbestosis ( = 19) than in those with no fibrotic findings in HRCT following asbestos exposure ( = 66) or in unexposed healthy controls. In asbestos-exposed subjects, plasma YKL-40 correlated negatively with lung function capacity parameters FVC (Pearson's -0.259, = 0.018) and FEV (Pearson's -0.240, = 0.028) and positively with CRP (Spearman's rho 0.371, < 0.001), IL-6 (Spearman's rho 0.314, = 0.003), adipsin (Spearman's rho 0.459, < 0.001), and MMP-9 (Spearman's rho 0.243, = 0.025). The present finding suggests YKL-40 as a biomarker associated with fibrosis and inflammation in asbestos-exposed subjects.
YKL-40是一种由交替活化的巨噬细胞产生的几丁质酶样糖蛋白，与伤口愈合和纤维化相关。石棉沉着病是一种慢性石棉诱导的肺部疾病，其中上皮细胞损伤和肺泡巨噬细胞活化导致胶原蛋白生成增加和纤维化。我们研究了YKL-40是否与接触石棉沉着病的受试者的炎症、纤维化和/或肺功能有关。从85名中度或重度职业性石棉暴露男性和28名年龄匹配的健康对照者中采集静脉血样本。采用酶联免疫吸附测定（ELISA）法检测血浆YKL-40、CRP、IL-6、脂联素和MMP-9水平。石棉沉着病患者（n = 19）的血浆YKL-40水平显著高于石棉暴露后HRCT无纤维化表现的患者（n = 66）或未暴露的健康对照者。在石棉暴露受试者中，血浆YKL-40与肺功能参数FVC（Pearson相关系数r = -0.259，P = 0.018）和FEV（Pearson相关系数r = -0.240，P = 0.028）呈负相关，与CRP（Spearman秩相关系数rho = 0.371，P < 0.001）、IL-6（Spearman秩相关系数rho = 0.314，P = 0.003）、脂联素（Spearman秩相关系数rho = 0.459，P < 0.001）和MMP-9（Spearman秩相关系数rho = 0.243，P = 0.025）呈正相关。目前的研究结果表明，YKL-40是与石棉暴露受试者的纤维化和炎症相关的生物标志物。
