Inhibition of -Amylases by Condensed and Hydrolysable Tannins: Focus on Kinetics and Hypoglycemic Actions.

The aim of the present study was to compare the in vitro inhibitory effects on the salivary and pancreatic -amylases and the in vivo hypoglycemic actions of the hydrolysable tannin from Chinese natural gall and the condensed tannin from The human salivary -amylase was more strongly inhibited by the hydrolysable than by the condensed tannin, with the concentrations for 50% inhibition (IC) being 47.0 and 285.4 M, respectively. The inhibitory capacities of both tannins on the pancreatic -amylase were also different, with IC values being 141.1 M for the hydrolysable tannin and 248.1 M for the condensed tannin. The kinetics of the inhibition presented complex patterns in that for both inhibitors more than one molecule can bind simultaneously to either the free enzyme of the substrate-complexed enzyme (parabolic mixed inhibition). Both tannins were able to inhibit the intestinal starch absorption. Inhibition by the hydrolysable tannin was concentration-dependent, with 53% inhibition at the dose of 58.8 mol/kg and 88% inhibition at the dose of 294 mol/kg. For the condensed tannin, inhibition was not substantially different for doses between 124.4 mol/kg (49%) and 620 mol/kg (57%). It can be concluded that both tannins, but especially the hydrolysable one, could be useful in controlling the postprandial glycemic levels in diabetes.