Department of Neurosciences and Behavioral Sciences, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, SP, Brazil.
Department of Physiological Sciences, Federal University of São Carlos, São Carlos, SP, Brazil.
Pituitary. 2017 Oct;20(5):515-521. doi: 10.1007/s11102-017-0812-z.
BACKGROUND/PURPOSE: Recent studies have reported that sepsis survivors show impaired central nervous system functions. The osmoregulation in this post-sepsis condition has not been well investigated. In the present study, we evaluated the secretion of neurohypophyseal hormones, arginine vasopressin (AVP) and oxytocin (OT), and water intake induced by osmotic challenge in survivor rats.
Wistar rats were submitted to sepsis by cecal ligation and puncture (CLP). Five days after CLP surgery, the survivor and naive animals were stimulated with an osmotic challenge consisting of hypertonic saline administration. Thirty minutes later, blood and brain were collected for determination of osmolality, nitrite, interleukin (IL)-1β, IL-6, AVP and OT levels and c-fos expression analysis of hypothalamic supraoptic nuclei (SON), respectively. In another set of sepsis survivor animals, water intake was measured for 240 min after the osmotic stimulus.
High levels of nitrite and IL-1β, but not IL-6, were found in the plasma of sepsis survivors and this long-term systemic inflammation was not altered by the osmotic challenge. Moreover, the AVP and OT secretion (but not the osmolality) and c-fos expression in SON were significantly attenuated in CLP survivor animals. Additionally, there was no alteration in the water intake response induced by osmotic challenge in the sepsis survivor group.
The results suggest that the inflammatory components mediated a persistent impairment in the component of the osmoregulatory reflex affecting the secretion of neurohypophyseal hormones in sepsis survivor animals.
背景/目的：最近的研究报告表明，脓毒症幸存者表现出中枢神经系统功能受损。这种脓毒症后的渗透压调节尚未得到充分研究。在本研究中，我们评估了存活大鼠在渗透压刺激下神经垂体激素（AVP 和 OT）的分泌和水摄入。
Wistar 大鼠通过盲肠结扎和穿孔（CLP）进行脓毒症。CLP 手术后 5 天，幸存者和未处理动物接受高渗盐水给药的渗透压刺激。30 分钟后，收集血液和大脑，分别用于测定渗透压、亚硝酸盐、白细胞介素 (IL)-1β、IL-6、AVP 和 OT 水平以及下丘脑视上核 (SON) 的 c-fos 表达分析。在另一组脓毒症幸存者动物中，在渗透压刺激后测量 240 分钟的水摄入量。
脓毒症幸存者的血浆中发现亚硝酸盐和 IL-1β水平升高，但 IL-6 水平没有升高，这种长期全身炎症未被渗透压刺激改变。此外，CLP 幸存者动物的 AVP 和 OT 分泌（但不是渗透压）和 SON 中的 c-fos 表达明显减弱。此外，在脓毒症幸存者组中，渗透压刺激引起的水摄入反应没有改变。
结果表明，炎症成分介导了对影响脓毒症幸存者动物神经垂体激素分泌的渗透压反射成分的持续损害。
