Ulbricht Carolin, Lindquist Randall L, Tech Laura, Hauser Anja E
Immune Dynamics and Intravital Microscopy, Charité Universitätsmedizin, Charitéplatz 1, 10117, Berlin, Germany.
Deutsches Rheumaforschungszentrum, Charitéplatz 1, 10117, Berlin, Germany.
Methods Mol Biol. 2017;1623:37-50. doi: 10.1007/978-1-4939-7095-7_3.
Due to the multitude of cell types involved in the differentiation of plasma cells during the germinal center reaction, and due to a lack of in vitro systems, which recapitulate germinal centers, the most suitable way to study plasma cell generation in germinal centers is in vivo. In this chapter we describe how to induce humoral immune responses to defined model antigens and how to visualize and track plasma cells and their interactions with other cells in the lymph nodes of living mice.
由于生发中心反应期间浆细胞分化涉及多种细胞类型，且缺乏能重现生发中心的体外系统，因此研究生发中心中浆细胞生成的最合适方法是在体内进行。在本章中，我们描述了如何诱导对特定模型抗原的体液免疫反应，以及如何在活小鼠的淋巴结中可视化和追踪浆细胞及其与其他细胞的相互作用。
