Judd A M, Koike K, MacLeod R M
Am J Physiol. 1985 Apr;248(4 Pt 1):E438-42. doi: 10.1152/ajpendo.1985.248.4.E438.
Arachidonate and its metabolites increase growth hormone release in vitro. A study was designed to determine whether arachidonate release from anterior pituitary cells is modified by growth hormone-releasing factor (GRF) or somatostatin (SRIF). Cultured pituitary cells were incubated with [3H]arachidonate to esterify the long-chain fatty acid into cellular lipids. The cells were extensively washed with medium containing no [3H]arachidonate and then incubated with GRF and/or SRIF for 30 min. The incubation medium was then extracted with ethyl acetate, and following thin-layer chromatographic separation, the radioactivity in the [3H]arachidonate band was measured. GRF in a concentration-dependent manner (1-30 nM) stimulated growth hormone and arachidonate release, whereas SRIF (100 nM) blocked the GRF-induced increase of growth hormone and arachidonate release. The effects of GRF on growth hormone and arachidonate were evident at time intervals as brief as 5 min. These findings support the hypothesis that arachidonate may play a role in the GRF-induced growth hormone release.
花生四烯酸及其代谢产物在体外可增加生长激素的释放。本研究旨在确定生长激素释放因子（GRF）或生长抑素（SRIF）是否会改变垂体前叶细胞中花生四烯酸的释放。将培养的垂体细胞与[3H]花生四烯酸一起孵育，以使长链脂肪酸酯化到细胞脂质中。用不含[3H]花生四烯酸的培养基对细胞进行充分洗涤，然后与GRF和/或SRIF一起孵育30分钟。然后用乙酸乙酯萃取孵育培养基，经薄层层析分离后，测量[3H]花生四烯酸条带中的放射性。GRF以浓度依赖性方式（1 - 30 nM）刺激生长激素和花生四烯酸的释放，而SRIF（100 nM）则阻断GRF诱导的生长激素和花生四烯酸释放的增加。GRF对生长激素和花生四烯酸的作用在短至5分钟的时间间隔内就很明显。这些发现支持了花生四烯酸可能在GRF诱导的生长激素释放中起作用的假说。
