Reyl-Desmars F, Baird A, Zeytin F N
Biochem Biophys Res Commun. 1985 Mar 29;127(3):977-85. doi: 10.1016/s0006-291x(85)80040-1.
The effect of GRF adenylate cyclase activation was studied in normal human, bovine and rat pituitary tissues. Human GRF (hGRF) activates adenylate cyclase in normal human pituitary membrane preparations in a concentration dependent manner (ED5 0 = 10(-11) M). In bovine pituitary cells hGRF stimulates GH secretion into the medium (ED5 0 = 7 X 10(-12) M) and activates adenylate cyclase (ED5 0 = 10(-11) M). In normal rat pituitary cells in monolayer culture, rat GRF (rGRF) stimulates adenylate cyclase (ED5 0 = 3 X 10(-11) M). In normal human pituitary membrane preparations and in normal rat pituitary cells in culture, somatostatin inhibits GRF-stimulated adenylate cyclase in a non-competitive manner, while it does not affect basal (i.e. non-stimulated) adenylate cyclase levels. VIP, a peptide which is structurally homologous to hGRF and rGRF is a weak GRF-agonist and activates adenylate cyclase in human and rat pituitary preparations at concentrations greater than 10 nM.
在正常人类、牛和大鼠的垂体组织中研究了生长激素释放因子（GRF）对腺苷酸环化酶的激活作用。人GRF（hGRF）以浓度依赖性方式激活正常人垂体膜制剂中的腺苷酸环化酶（半数有效剂量[ED50]=10^(-11)M）。在牛垂体细胞中，hGRF刺激生长激素分泌到培养基中（ED50=7×10^(-12)M）并激活腺苷酸环化酶（ED50=10^(-11)M）。在单层培养的正常大鼠垂体细胞中，大鼠GRF（rGRF）刺激腺苷酸环化酶（ED50=3×10^(-11)M）。在正常人垂体膜制剂和培养的正常大鼠垂体细胞中，生长抑素以非竞争性方式抑制GRF刺激的腺苷酸环化酶，而不影响基础（即未刺激）的腺苷酸环化酶水平。血管活性肠肽（VIP）是一种在结构上与hGRF和rGRF同源的肽，是一种弱GRF激动剂，在浓度大于10 nM时激活人和大鼠垂体制剂中的腺苷酸环化酶。
