Rodríguez María Magdalena, Crespo Ariel, Hurtado Daymi, Fuentes Ilario, Rey Jorge, Bisset Juan Andrés
J Am Mosq Control Assoc. 2017 Jun;33(2):142-144. doi: 10.2987/16-6593.1.
The objective of this study was to determine diagnostic doses (DDs) of 5 insecticides for the Rockefeller susceptible strain of Aedes aegypti , using the Centers for Disease Control and Prevention (CDC) bottle bioassay as a tool for monitoring insecticide resistance in the Cuban vector control program. The 30-min DD values determined in this study were 13.5 μg/ml, 6.5 μg/ml, 6 μg/ml, 90.0 μg/ml, and 15.0 μg/ml for cypermethrin, deltamethrin, lambda-cyhalothrin, chlorpyrifos, and propoxur, respectively. To compare the reliability of CDC bottle bioassay with the World Health Organization susceptible test, 3 insecticide-resistant strains were evaluated for deltamethrin and lambda-cyhalothrin. Results showed that the bottles can be used effectively from 21 to 25 days after treatment and reused up to 4 times, depending on the storage time. The CDC bottle bioassay is an effective tool to assess insecticide resistance in field populations of Ae. aegypti in Cuba and can be incorporated into vector management programs using the diagnostic doses determined in this study.
本研究的目的是利用美国疾病控制与预防中心（CDC）的瓶式生物测定法作为古巴病媒控制项目中监测杀虫剂抗性的工具，确定5种杀虫剂对洛克菲勒敏感品系埃及伊蚊的诊断剂量（DDs）。本研究确定的30分钟诊断剂量值，氯氰菊酯为13.5μg/ml、溴氰菊酯为6.5μg/ml、高效氯氟氰菊酯为6μg/ml、毒死蜱为90.0μg/ml、残杀威为15.0μg/ml。为比较CDC瓶式生物测定法与世界卫生组织敏感试验的可靠性，对3个抗杀虫剂品系进行了溴氰菊酯和高效氯氟氰菊酯评估。结果表明，处理后21至25天内瓶式生物测定法可有效使用，根据储存时间，可重复使用多达4次。CDC瓶式生物测定法是评估古巴埃及伊蚊野外种群杀虫剂抗性的有效工具，可利用本研究确定的诊断剂量纳入病媒管理项目。
