Wang Xin, Fu Jian-Hua, Xue Xin-Dong
MD, Department of Pediatrics, Shengjing Hospital, China Medical University, Shenyang, China. Conception and design of the study, technical procedures, acquisition and analysis of data, manuscript writing.
MD, Department of Pediatrics, Shengjing Hospital, China Medical University, Shenyang, China. Analysis and interpretation of data, manuscript writing.
Acta Cir Bras. 2017 May;32(5):359-368. doi: 10.1590/s0102-865020170050000005.
: To evaluate the changes of caveolin-1 in lung fibroblasts in newborn Wistar rats when exposed to hyperoxic conditions, as well as lung fibroblasts cell cycle.
: One hundred newborn Wistar rats were randomly divided (50 rats/group) into experimental and control groups, exposed to hyperoxic conditions or normal air, respectively. The fraction of inspired oxygen (FiO2) in the experimental group was 90%, whereas this value was 21% in the control group. Lung fibroblasts were collected on days 3, 7, and 14 of the experiment. Caveolin-1 expression dynamics in lung fibroblasts was assayed in each group by immunofluorescence and Western blot analyses. Flow cytometry (FCM) was used to assess the proportions of lung fibroblasts at different stages of the cell cycle.
: On day 3, no significant difference in caveolin-1 expression was observed between the hyperoxic and control groups; however, on days 7 and 14, caveolin-1 expression was significantly lower in the hyperoxic group than in the control (P<0.05). No apparent differences were observed in caveolin-1 expression in the control group at the different time points. Using FCM analysis, we showed that the proportion of lung fibroblasts in G0/G1 phase in the hyperoxic group decreased compared to that of the control group on day 7, while the proportion of S-phase cells increased (P<0.05). These differences were more significant when the groups were compared on day 14 (P<0.01).
: After seven days the exposure to hyperoxic conditions, lung fibroblasts proliferated and caveolin-1 expression decreased.
评估新生Wistar大鼠肺成纤维细胞在高氧环境下小窝蛋白-1（caveolin-1）的变化以及肺成纤维细胞的细胞周期。
将100只新生Wistar大鼠随机分为两组（每组50只），分别置于高氧环境或正常空气中。实验组吸入氧分数（FiO2）为90%，而对照组为21%。在实验的第3、7和14天收集肺成纤维细胞。通过免疫荧光和蛋白质印迹分析检测每组肺成纤维细胞中caveolin-1的表达动态。采用流式细胞术（FCM）评估肺成纤维细胞在细胞周期不同阶段的比例。
第3天，高氧组和对照组之间caveolin-1表达无显著差异；然而，在第7天和第14天，高氧组中caveolin-1表达显著低于对照组（P<0.05）。对照组在不同时间点的caveolin-1表达未观察到明显差异。通过FCM分析，我们发现与对照组相比，高氧组在第7天时G0/G1期肺成纤维细胞比例降低，而S期细胞比例增加（P<0.05）。在第14天比较两组时，这些差异更为显著（P<0.01）。
暴露于高氧环境7天后，肺成纤维细胞增殖且caveolin-1表达降低。
