Bartoš Vladimír, Kullová Milada
Department of Pathology, Faculty Hospital in Žilina, V. Spanyola 43, Žilina, 012 07, Slovakia.
Department of Dermatovenerology, Faculty Hospital in Žilina, V. Spanyola 43, Žilina, Slovakia.
Acta Medica (Hradec Kralove). 2017;60(1):32-36. doi: 10.14712/18059694.2017.48.
P120(ctn) is a specific membranous adhesion protein, that maintains the stability of intercellular junctions. An altered expression of p120(ctn), either reduced in the cell membrane or increase in the cytoplasm, plays a crucial role in carcinogenesis. No research has analysed the expression of p120(ctn) in basal cell carcinoma (BCC) of the skin so far. Therefore, we immunohistochemically studied p120(ctn) in a set of cutaneous BCCs in order to determine, whether there is difference in the expression pattern related to the histologic subtypes and tumor growth characteristics.
The study group consisted of 38 BCCs cathegorized into low-risk (non-infiltrative) subroup (8 superficial and 12 nodular subtypes) and high-risk (infiltrative) subgroup (10 nodular-infiltrative and 8 infiltrative subtypes). Specific monoclonal antibody against p120(ctn) was used for staining.
Overall, there were 12 cases (31.6%) with normal preserved and 26 cases (68.4%) with abnormal p120(ctn) expression. In superficial, nodular, nodular-infiltrative and infiltrative subtypes, abnormal p120(ctn) immunoreactivity was found in 37.5% (3/8), 41.7% (5/12), 100% (10/10) and 100% (8/8), respectively. We have confirmed a strong correlation between the expression of p120(ctn) and both given, non-infiltrative and infiltrative BCC growth phenotypes. In the latter subgroup, almost all lesions showed diffusely reduced membranous staining, of which five also manifested an aberrant immunoreactivity in the cytoplasm. This cytoplasmic positivity occurred solely at the invasive front of the infiltrative tumor formations.
Our results showed that decreased membranous expression of p120(ctn) was a frequent event in human cutaneous BCC and it was associated with infiltrative growth phenotype. Considering that nearly half of the BCCs with non-infiltrative growth pattern also exhibited reduced membranous expression, aberrant cytoplasmic immunoreactivity of p120(ctn), which was found exclusively in the high-risk BCC variants, can more reliably reflect and predict biological behaviour and malignant potential.
P120（连环蛋白）是一种特定的膜黏附蛋白，可维持细胞间连接的稳定性。P120（连环蛋白）表达改变，无论是细胞膜上表达降低还是细胞质中表达增加，在肿瘤发生过程中都起着关键作用。目前尚无研究分析P120（连环蛋白）在皮肤基底细胞癌（BCC）中的表达情况。因此，我们采用免疫组织化学方法研究了一组皮肤BCC中P120（连环蛋白）的表达，以确定其表达模式与组织学亚型及肿瘤生长特征是否存在差异。
研究组由38例BCC组成，分为低风险（非浸润性）亚组（8例浅表型和12例结节型）和高风险（浸润性）亚组（10例结节浸润型和8例浸润型）。使用针对P120（连环蛋白）的特异性单克隆抗体进行染色。
总体而言，12例（31.6%）P120（连环蛋白）表达正常，26例（68.4%）表达异常。在浅表型、结节型、结节浸润型和浸润型亚型中，P120（连环蛋白）免疫反应异常分别见于37.5%（3/8）、41.7%（5/12）、100%（10/10）和100%（8/8）。我们证实了P120（连环蛋白）的表达与给定的非浸润性和浸润性BCC生长表型之间存在密切相关性。在后一亚组中，几乎所有病变均显示膜染色弥漫性降低，其中5例在细胞质中也表现出异常免疫反应。这种细胞质阳性仅出现在浸润性肿瘤形成的浸润前沿。
我们的结果表明，P120（连环蛋白）膜表达降低在人类皮肤BCC中很常见，且与浸润性生长表型相关。鉴于近一半非浸润性生长模式的BCC也表现出膜表达降低，P120（连环蛋白）异常的细胞质免疫反应仅在高风险BCC变体中发现，能更可靠地反映和预测生物学行为及恶性潜能。
