Bartoš Vladimír, Kullová Milada
Vladimír Bartoš, MD, PhD, MSc, Björnsonova 3/5 , Martin, 036 01, Slovakia;
Acta Dermatovenerol Croat. 2015;23(4):254-9.
Calponin is an actin filament-associated protein significantly involved in the regulation of the cellular motility. Some data have indicated that overproduction of calponin in basal cell carcinoma (BCC) of the skin may be responsible for local tumor invasiveness and more aggressive biological behavior. We studied the immunohistochemical expression of calponin in a set of cutaneous BCCs, in order to clarify whether the presence of calponin in cancer cells may be a predictor of invasive tumor growth. The study group consisted of 37 primary BCCs categorized into a non-infiltrative subgroup (5 superficial, 16 nodular subtypes) and infiltrative subgroup (9 nodular-infiltrative, 7 infiltrative subtypes). A specific monoclonal antibody against calponin was used for staining. Expression of calponin in tumor tissue was found in 72.9% (27/37) of the cases, though staining intensity was relatively weak. In superficial, nodular, nodular-infiltrative, and infiltrative BCC subtypes, calponin positivity was found in 80% (4/5), 75% (12/16), 66.7% (6/9), and 71.5% (5/7), respectively. We did not confirm a significant correlation between expression of calponin and given, non-infiltrative, and infiltrative BCC subgroups. Furthermore, we found seven BCCs (18.9%) with striking immunoreactivity for calponin in adjacent peritumorous stroma. There was a significant association between stromal immunoreactivity for calponin and tumor growth histomorphology being positive only in BCCs with infiltrative growth features. Our study has shown that neoplastic cells in cutaneous BCC commonly produce calponin regardless of histological subtype. Expression of calponin in tumor tissue was not associated with the aggressive tumor phenotype. However, since some BCCs with infiltrative growth patterns strongly expressed calponin in peritumorous stroma, this finding could more reliably reflect the biological behavior of cancer and should be better explained in the future.
钙调蛋白是一种与肌动蛋白丝相关的蛋白质，在细胞运动调节中发挥着重要作用。一些数据表明，皮肤基底细胞癌（BCC）中钙调蛋白的过度产生可能与局部肿瘤侵袭性及更具侵袭性的生物学行为有关。我们研究了一组皮肤基底细胞癌中钙调蛋白的免疫组化表达情况，以明确癌细胞中钙调蛋白的存在是否可作为肿瘤侵袭性生长的预测指标。研究组由37例原发性基底细胞癌组成，分为非浸润性亚组（5例浅表型、16例结节型）和浸润性亚组（9例结节浸润型、7例浸润型）。使用一种针对钙调蛋白的特异性单克隆抗体进行染色。在72.9%（27/37）的病例中发现肿瘤组织中有钙调蛋白表达，不过染色强度相对较弱。在浅表型、结节型、结节浸润型和浸润型基底细胞癌亚型中，钙调蛋白阳性率分别为80%（4/5）、75%（12/16）、66.7%（6/9）和71.5%（5/7）。我们未证实钙调蛋白表达与特定的非浸润性和浸润性基底细胞癌亚组之间存在显著相关性。此外，我们发现7例基底细胞癌（18.9%）在相邻的肿瘤周围基质中对钙调蛋白有显著的免疫反应性。钙调蛋白的基质免疫反应性与肿瘤生长组织形态学之间存在显著关联，仅在具有浸润性生长特征的基底细胞癌中呈阳性。我们的研究表明，皮肤基底细胞癌中的肿瘤细胞通常会产生钙调蛋白，而与组织学亚型无关。肿瘤组织中钙调蛋白的表达与侵袭性肿瘤表型无关。然而，由于一些具有浸润性生长模式的基底细胞癌在肿瘤周围基质中强烈表达钙调蛋白，这一发现可能更可靠地反映癌症的生物学行为，未来应进一步深入阐释。
