State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.
Synergistic Innovation Center for Food Safety and Nutrition, Jiangnan University, Wuxi, 214122, China.
Sci Rep. 2017 Jun 7;7(1):2929. doi: 10.1038/s41598-017-03231-9.
All-trans retinoic acid (ATRA), one of vitamin A derivatives, shows greater growth inhibition of breast cancer cell for ER-positive than ER-negative cells, while triple negative breast cancer cell such as MDA-MB-231 cell is poorly responsive to ATRA treatment. In this study, we found that combination of ω-3 free fatty acids (ω-3 FFAs) and ATRA exhibited synergistic inhibition of cell growth in three subtypes (ER MCF7, HER2 SK-BR-3, Triple negative HCC1806 and MDA-MB-231 cells) of human breast cancer cell lines. The combined treatment of ω-3 FFAs and ATRA resulted in cell cycle arrest. ω-3 FFAs combined with ATRA synergistically provoked cell apoptosis via the caspase signals but not p53. These findings suggest that combined chemotherapy of ω-3 FFAs with ATRA is beneficial for improvement of ATRA sensitivity in breast cancer cells.
全反式视黄酸（ATRA）是维生素 A 衍生物之一，对 ER 阳性乳腺癌细胞的生长抑制作用大于 ER 阴性细胞，而三阴性乳腺癌细胞（如 MDA-MB-231 细胞）对 ATRA 治疗反应不佳。在这项研究中，我们发现 ω-3 游离脂肪酸（ω-3 FFAs）与 ATRA 的联合使用在三种人乳腺癌细胞系（ER MCF7、HER2 SK-BR-3、三阴性 HCC1806 和 MDA-MB-231 细胞）中表现出协同抑制细胞生长的作用。ω-3 FFAs 与 ATRA 的联合治疗导致细胞周期停滞。ω-3 FFAs 与 ATRA 联合协同通过 caspase 信号而不是 p53 引发细胞凋亡。这些发现表明，ω-3 FFAs 与 ATRA 的联合化疗有利于提高乳腺癌细胞对 ATRA 的敏感性。
