Malešević Milka, Vasiljević Zorica, Sovtić Aleksandar, Filipić Brankica, Novović Katarina, Kojić Milan, Jovčić Branko
Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, P.O. Box 23, Belgrade, 11010 Serbia.
Department of Clinical Microbiology, Mother and Child Health Care Institute of Serbia "Dr. Vukan Cupic", Radoja Dakica 8, Belgrade, 11070 Serbia.
Antimicrob Resist Infect Control. 2017 Jun 6;6:57. doi: 10.1186/s13756-017-0215-y. eCollection 2017.
is considered one of the most problematic cystic fibrosis (CF) pathogens. Colonization prevalence in the Serbian CF population is high and virtually exclusively limited to a single highly transmissible clone of ST856 which is positive for both the epidemic strain marker (BCESM) and cable pilin, and is closely related to the epidemic strain CZ1 (ST32).
Biofilm formation for 182 isolates, and adhesion to components of the host extracellular matrix, proteolytic activity, mucoidy and motility of selected ST856 representatives, as well as ST858 and ST859, and ST857, novel STs isolated from Serbian CF patients, were investigated in this study. The presence of the , , , , , and genes was analyzed.
Biofilm-formation ability of analyzed strains was poor under standard laboratory conditions, but changed in stress conditions (cold stress) and conditions that mimic CF milieu (increased CO). All strains expressed ability to bind to collagen and fibronectin albeit with different intensity. Representatives of ST856 exhibited gelatinase activity. ST858, ST859 and 9/11 of ST856 genotypes were positive for swimming and twitching motility whereas ST857 was non-motile. Mucoidy was demonstrated in all ST856 genotypes, ST857 was semi-mucoid, and ST858 and ST859 were non-mucoid. Molecular analysis for major virulence factors revealed that ST856 and ST857 carried the six analyzed genes, while ST858 and ST859 were negative for the gene.
Variations in virulence phenotypes in different genotypes of epidemic ST856 clone, in vitro, could be a consequence of diversification driven by pathoadaptation. Diversity of epidemic clone genotypes virulence, could be challenging for accurate diagnosis and treatment, as well as for infection control.
被认为是囊性纤维化（CF）最具问题的病原体之一。在塞尔维亚CF患者群体中的定植率很高，且几乎完全局限于单一高度可传播的ST856克隆，该克隆对流行菌株标志物（BCESM）和电缆菌毛蛋白均呈阳性，并且与流行菌株CZ1（ST32）密切相关。
本研究调查了182株分离株的生物膜形成、对宿主细胞外基质成分的粘附、蛋白水解活性、所选ST856代表株以及从塞尔维亚CF患者中分离出的新STs即ST858、ST859和ST857的黏液样性和运动性。分析了、、、、和基因的存在情况。
在标准实验室条件下，所分析菌株的生物膜形成能力较差，但在应激条件（冷应激）和模拟CF环境的条件（二氧化碳增加）下会发生变化。所有菌株均表现出与胶原蛋白和纤连蛋白结合的能力，尽管强度不同。ST856代表株表现出明胶酶活性。ST858、ST859和ST856基因型的9/11具有游泳和颤动运动能力，而ST857无运动能力。所有ST856基因型均表现出黏液样性，ST857为半黏液样，ST858和ST859为非黏液样。主要毒力因子的分子分析显示，ST856和ST857携带所分析的6个基因，而ST858和ST859的基因呈阴性。
流行的ST856克隆不同基因型在体外毒力表型的差异可能是由致病适应驱动的多样化的结果。流行克隆基因型毒力的多样性可能对准确诊断和治疗以及感染控制构成挑战。
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